一个染色体层次的基因组组合的Corcyra cephalonica Stainton (Lepidoptera: Pyralidae) 的
Yinhuan Ding1, Jianfeng Jin2,3, Mingli Fang1
1Department of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang, Jurong, 212400, China.
Scientific data
|October 11, 2024
概括
对一个重要的储存产品害虫米 (Corcyra cephalonica) 的染色体级基因组进行了测序. 这种高质量的基因组为了解其生态和生物学提供了基础.
科学领域:
- 基因组学就是基因组学.
- 昆虫学 昆虫学是一门学科.
- 虫害管理 虫害管理 虫害管理
背景情况:
- 科 (Pyralidae) 家庭,包括米Corcyra cephalonica,包括5900多个物种,具有生态意义.
- 科尔西拉 (Corcyra cephalonica) 是储存谷物和产品的主要害虫,影响粮食安全.
- 对C. cephalonica的有限基因组数据限制了全面的生态和生物研究.
研究的目的:
- 为了生成一个高质量的,染色体水平的基因组组合Corcyra cephalonica.
- 为未来关于C. cephalonica生物学和害虫管理策略的研究提供基础的基因组资源.
主要方法:
- 长读 (纳米孔) 和短读 (Illumina) 测序数据的整合.
- 利用Hi-C技术进行染色体支架和组装.
- 包括BUSCO在内的生物信息分析用于评估基因组完整性和重复元素识别.
主要成果:
- 成功生成了453.37Mb的染色体级基因组组,定在29个染色体上.
- 该组件具有高连续性,采用16.67 Mb的N50脚手架和99.0%的BUSCO完整性.
- 确定了14135个蛋白质编码基因和48.74%的基因组内重复元素.
结论:
- 科尔西拉·塞法洛尼卡 (Corcyra cephalonica) 的染色体层次基因组代表了Lepidopteran基因组学的重大进步.
- 这种基因组资源将促进对物种进化生物学,害虫行为和控制的深入调查.
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