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相关概念视频

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

Updated: Jun 10, 2025

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy
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一次射击20倍膨胀显微镜.

Shiwei Wang1,2, Tay Won Shin1,3, Harley B Yoder1,4,5

  • 1McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, USA.

Nature methods
|October 11, 2024
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概括

我们开发了20ExM,这是一种新的扩展显微镜 (ExM) 技术. 这种单步协议在传统显微镜上实现了纳米尺度成像的~20×物理放大.

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科学领域:

  • 生物技术是生物技术.
  • 显微镜的使用方法
  • 细胞生物学 细胞生物学

背景情况:

  • 扩展显微镜 (ExM) 允许使用传统显微镜进行纳米尺度成像.
  • 目前的ExM方法提供有限的扩展 (~4-10×) 或需要代步骤来获得更高的放大 (~15-20×).

研究的目的:

  • 引入一个简化的ExM协议,在单个步骤中实现高线性扩展.
  • 用标准显微镜设备实现纳米级分辨率 (<20 nm).

主要方法:

  • 开发了一种新的单步扩展显微镜协议,称为20ExM.
  • 实现了生物标本的约20×线性物理放大.

主要成果:

  • 20ExM协议在传统显微镜上提供<20nm分辨率.
  • 这种方法简化了高放大ExM,在性能上与代方法相匹配.
  • 20ExM支持扩张后染色,特别是对于大脑组织.

结论:

  • 20ExM为高分辨率纳米尺度成像提供了一步解决方案.
  • 该协议促进了生物分子标签,并在生物研究中广泛应用.
  • 这种技术增强了传统显微镜用于先进成像的实用性.