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对调节Cdkn1c (p57基因) 表达的增强元件的鉴定和表征

Daisuke Koga¹, Shogo Nakayama¹, Tsunaki Higa^1,2

¹Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

Genes to cells : devoted to molecular & cellular mechanisms

|October 12, 2024

概括

研究人员确定了小鼠p57基因 (Cdkn1c) 的新增增强器区域. 这种位于Kcnq1位点内的调节元件对于胚胎发育期间的Cdkn1c表达至关重要.

科学领域:

发展生物学发展生物学
基因规则基因规则
分子遗传学分子遗传学

背景情况:

蛋白质p57 (由Cdkn1c基因编码) 是一种循环素依赖的激酶抑制剂,对哺乳动物胚胎发生和成体干细胞维护至关重要.
虽然调节Cdkn1c的转录因子已知,但特定的cis-regulatory元素,如增强剂尚未得到很好的定义.

研究的目的:

识别和描述控制小鼠p57基因 (Cdkn1c) 表达的cis调节元件,特别是增强剂.

主要方法:

在小鼠胚胎干细胞 (mESCs) 中利用4C-seq分析来识别潜在的增强器区域.
使用CRISPR-Cas9基因编辑进行删除和CRISPR干扰 (CRISPRi) 抑制候选增强器区域.

主要成果:

在Kcnq1位点的内tron中确定了Cdkn1c基因的候选增强器区域.
删除或抑制该区域导致Cdkn1c表达在分化mESCs中的显著下降.

结论:

鉴定到的内基区域作为小鼠p57 (Cdkn1c) 基因的关键增强剂.
这种增强剂在调节Cdkn1c在小鼠早期胚胎发生过程中的表达方面发挥着重要作用.

关键词:

胚胎干细胞是一种胚胎干细胞.增强剂是一种增强剂.基因表达的基因表达方式在p5757中,我们可以看到

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