[通过感应合等离子体质谱测定全血中的22个元素]
1Chemical Poisoning and Heavy Metal Detection Center, Guangxi Zhuang Autonomous Region Institute for the Prevention and Treatment of Occupational Disease, Nanning 530021, China.
概括
一种新的感应合血质谱法 (ICP-MS) 方法可以准确地确定全血中的22个元素. 这种快速而简单的技术适用于临床的多元元素血液分析.
科学领域:
- 分析化学 分析化学
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
背景情况:
- 准确的全血元素分析对于健康监测至关重要.
- 现有的方法可能缺乏用于全面元素分析的灵敏度或吞吐量.
研究的目的:
- 开发和验证一种用于量化全血中22个元素的同时方法.
- 使用感应合等离子体质谱法 (ICP-MS) 进行高通量元素分析.
主要方法:
- 优化ICP-MS参数,样品预处理和稀释因子.
- 整个血液样本被稀释和离心;通过ICP-MS分析的超级生物.
- 方法验证包括对线性,检测极限,精度和恢复的评估.
主要成果:
- 在所有22个元素中观察到出色的线性 (r=0.9991-0.9999).
- 低检测极限范围从0.003μg/L到0.012 mg/L之间.
- 实现了高精度 (集团内部:0.5%-7.2%,集团间:0.4%-9.4%) 和回收 (80.6%-114.9%).
结论:
- 开发的ICP-MS方法可以准确和精确地同时确定全血中的22个元素.
- 该方法快速,简单,适合常规临床应用.
- 这种技术有助于对健康状况进行全面的基本分析.
相关概念视频
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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
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AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
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Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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