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在受 Staphylococcus aureus 伤害的牛乳腺上皮细胞中,N6 - - 甲基氨酸修饰的 lncRNA

Haojun Xu^1,2, Xuan Wu^1,2, Zhiming Yang²

¹Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

Archives of microbiology

|October 12, 2024

概括

这项研究确定了新型m6A-修饰的长非编码RNA (lncRNAs) 参与由黄金葡萄球菌引起的牛乳腺炎. 这些发现为治疗这种重要的乳牛疾病提供了新的表观遗传点.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学
兽医科学兽医科学兽医科学

背景情况:

黄金葡萄球菌引起的乳腺炎是乳牛的重大挑战,缺乏有效的治疗方法.
对乳腺炎的抗生素使用有助于抗菌素耐药性,构成公共卫生风险.
长非编码RNAs (lncRNAs) 和m6A修饰与传染病有关,但它们在牛乳腺炎中的作用需要阐明.

研究的目的:

在牛乳腺上皮细胞中识别和表征m⁶A-修饰的lncRNAs在金黄色葡萄球菌感染期间.
调查这些修饰的 lncRNAs 的多样性和调控功能.

主要方法:

一个细胞损伤模型是使用热不活化的金黄色葡萄球菌建立的.
使者RNA免疫沉测序 (MeRIP-seq) 用于描述m6A修饰的lncRNAs.
进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.

主要成果:

黄金葡萄球菌诱导导致细胞活力下降和改变m6A修饰水平.
在140m⁶A修改峰值在130个lncRNA中在细菌挑战时观察到显著差异.
不同的m6A-修饰的lncRNAs主要在WNT途径中得到丰富,并参与氨基酸代谢,脂质转位和金属蛋白酶活性.

结论:

这项研究提供了第一份关于调节牛乳腺炎中黄金葡萄球菌感染的m⁶A-修饰的lncRNAs的报告.
这些发现突出了潜在的表观遗传机制和治疗点感染性疾病,如乳腺炎.

关键词:

黄金色的S. 黄金色的牛乳腺上皮细胞牛乳腺上皮细胞长非编码RNA是什么意思这是一种N6-甲基氨酸.

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...