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使用IP1同质时间解析光 (HTRF) 试验量化Gq信号

Aqfan Jamaluddin1,2

  • 1Metabolism and Systems Science, University of Birmingham, Birmingham, UK. a.jamaluddin@bham.ac.uk.

Methods in molecular biology (Clifton, N.J.)
|October 12, 2024
PubMed
概括

这项研究详细介绍了一种使用时间解析光共振能量转移 (TR-FRET) 来研究G蛋白结合受体 (GPCR) 信号通路的因诺酸-1 (IP1) 积累试验. 这种方法有助于对Gq结合受体进行药理学研究和化合物选.

关键词:
信号传递的.招募G蛋白质的方法IP3 IP3 IP3 IP3 IP3 IP3 IP3 IP3 IP3 IP3 IP3 IP3伊诺西托酸盐是一种酸盐.受体活动受体活动.二级信息传递器二级信息传递器TR-FRET测定试验的方法

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Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
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