相关实验视频

Updated: Aug 5, 2026

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

80.2K

通过TIRF显微镜测量感知受体激素驱动插入信号 (ADIS) 和贩运

Caroline M Gorvin1,2

  • 1Metabolism and Systems Science and Centre for Diabetes, Endocrinology and Metabolism (CEDAM), University of Birmingham, Birmingham, UK. c.gorvin@bham.ac.uk.

Methods in molecular biology (Clifton, N.J.)
|October 12, 2024
PubMed
概括

本研究详细介绍了一种方法来追踪感应受体 (CaSR) 运动,使用全内部反射光显微镜. 这种技术可以同时测量CaSR贩运和内细胞分裂,这对于了解平衡障碍至关重要.

关键词:
适应蛋白-2 的适应蛋白-2的恒常化是的恒常化.细胞内存症是指细胞内存症.过高/低血症的发生.甲状腺上腺激素激素的作用

更多相关视频

Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging10:05

Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging

Published on: June 20, 2016

7.6K
Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy08:55

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy

Published on: February 17, 2023

3.0K

相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.