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Updated: May 6, 2026

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
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使用ONE-GO生物传感器研究G蛋白结合受体信号动力学和度依赖反应的协议

Remi Janicot1, Mikel Garcia-Marcos2

  • 1Department of Biochemistry & Cell Biology, Chobanian & Avedisian School of Medicine, Boston University, Boston, MA 02118, USA.

STAR protocols
|October 12, 2024
PubMed
概括

这项研究引入了ONE向量G蛋白光学 (ONE-GO) 生物传感器,这是研究G蛋白结合受体 (GPCR) 活性的一种多功能工具. 该协议允许详细研究GPCR信号动力学和细胞系中的度反应.

关键词:
生物物理学的生物物理学.生物技术和生物工程 生物技术和生物工程细胞生物学 细胞生物学细胞培养培养的细胞培养.基于细胞的测定.分子生物学分子生物学信号传导的信号传导.

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G-protein Coupled Receptors01:21

G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
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