光探测无氧消化器污泥:用乳标记的拉曼分析测量甲基生物 (Methanosarcina和Methanothermobacter) 中的单细胞合成活性
Eustace Fernando1, Per Halkjær Nielsen2, Jeppe Lund Nielsen2
1Center for Microbial Communities, Department of Chemistry and Bioscience, Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark; Environmental Sustainability Laboratory, Department of Civil, Environmental and Ocean Engineering, Stevens Institute of Technology, Hoboken, NJ 07030. USA.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
|October 12, 2024
概括
这项研究引入了一种使用,FISH和拉曼光谱法测量单个微生物物种的新方法.
科学领域:
- 微生物生态学 微生物生态学
- 生物地质化学生物地质化学
- 分析化学 分析化学
背景情况:
- 了解单个微生物物种在复杂环境中的基质利用,如无氧消化器,对于过程优化至关重要.
- 目前的方法往往缺乏分辨率,以在微生物群落内在单细胞水平上区分代谢活动.
研究的目的:
- 开发和验证一种新的技术,用于量化特定微生物种群的 in situ 基质利用率.
- 为了在复杂的微生物联盟中实现单细胞代谢分析.
主要方法:
- 一种使用双标记,光现场杂交 (FISH) 和拉曼显微光谱的综合方法.
- 用拉曼光谱分析 (C-Dx/C-Hx峰值比率) 来量化的纳入微生物生物质.
- 为了识别,FISH探针针对特定的甲原性属 (Methanosarcina,Methanothermobacter).
主要成果:
- 该方法成功量化了的结合,表明在准的甲细胞中具有合成活性.
- 甲沙辛的代谢率与酸盐和酸盐的代谢率最高.
- 甲热菌表现出更快的基质与酸盐和甲醇的合并.
结论:
- 这种新-FISH-拉曼显微光谱方法为微生物群体提供了定量,单细胞代谢率数据.
- 该技术为在复杂的生态和工程系统中剖析微生物代谢提供了强大的工具.
相关概念视频
01:19Protein Dynamics in Living Cells
2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
03:31Labeling DNA Probes
8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K