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Updated: Jun 10, 2025

A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
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A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s

Published on: September 25, 2017

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选择溶剂和阳性对照度用于增强的Ames测试条件的N-尼托拉胺化合物

Chetan K Kajavadara1, Satyam N Patel1, Rushikesh M Shukla1

  • 1Department of Pharmacology and Toxicology, Zydus Research Center, Zydus Lifesciences Limited, Ahmedabad, 382213, India.

Regulatory toxicology and pharmacology : RTP
|October 12, 2024
PubMed
概括

这项研究为增强的艾姆斯测试 (EAT) 提供了明确的指导方针,以改善N-尼托拉胺变异原体的检测. 它解决了溶剂体积和阳性对照度的关键差距,以进行准确的致变性评估.

关键词:
1-环烯-4-尼托索皮佩拉.增强的艾姆斯试验条件代谢激活系统的代谢激活系统含有N-尼托胺的杂质这是一种N-二甲基胺.溶剂 溶剂 溶剂 是一种

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Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.
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