高葡萄糖诱导的衰老有助于糖尿病脏病患者的管状上皮细胞损伤
Deping Xu1, Puseletso Moru2, Kainan Liao2
1Department of Biochemistry and Molecular Biology, Anhui Medical University, Hefei, China; The Clinical Laboratory, the Affiliated Hefei Hospital of Anhui Medical University, Hefei Second People's Hospital, Hefei, China.
Experimental gerontology
|October 12, 2024
概括
高葡萄糖在糖尿病病 (DN) 中损害细胞,导致细胞衰老和上皮-介质细胞过渡 (EMT). 抗衰老化合物白醇减轻了这些有害影响,为DN提供了潜在的治疗益处.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 细胞生物学 细胞生物学
- 糖尿病学 糖尿病学
背景情况:
- 糖尿病病 (DN) 的特征是功能障碍的管状上皮细胞,通常与高葡萄糖水平有关.
- 高葡萄糖会诱导表皮层-介质酶过渡 (EMT),线粒体损伤,亡和管细胞中的细胞衰老,导致损伤.
- 高葡萄糖诱导的细胞衰老在加剧DN中的管状上皮细胞功能障碍中的精确作用需要进一步调查.
研究的目的:
- 调查EMT和高葡萄糖诱导的DN细胞衰老之间的关系.
- 检查高葡萄糖对管状上皮细胞的影响,重点关注线粒体功能障碍,衰老和亡.
- 评估复星对高葡萄糖诱导的DNA细胞损伤的保护作用.
主要方法:
- 使用了链毒素 (STZ) 诱导的糖尿病病 (DN) 鼠标模型和高葡萄糖 (HG) 治疗的HK-2细胞.
- 评估了表皮层-介质细胞过渡 (EMT),细胞衰老,线粒体功能障碍,亡,反应性氧物种 (ROS) 生产和细胞内Ca2+水平.
- 研究了白醇治疗对HG诱导的细胞损伤的影响.
主要成果:
- 高葡萄糖诱导的EMT,线粒体功能障碍,亡,增加ROS和内Ca2+在管细胞中.
- 细胞衰老被确定为DN中高葡萄糖暴露的重要后果.
- Resveratrol治疗有效地减轻了HG诱导的EMT,线粒体功能障碍,亡,并降低了ROS和Ca2+水平.
结论:
- 高葡萄糖诱导的细胞衰老和EMT是糖尿病脏病中管状上皮细胞功能障碍的关键贡献者.
- ресвератрол通过减轻EMT,线粒体功能障碍和亡,显示出对高葡萄糖诱导的细胞损伤的保护作用.
- 用像白醇这样的药物准细胞衰老和相关途径可能是治疗糖尿病病的治疗策略.
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