时间过程的转录组分析揭示了转录因子参与调节玉米中的敏感性
Mingliang Zhang1, Yuancong Wang1, Qi Wu1
1Institute of Crop Germplasm and Biotechnology, Jiangsu Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China.
Journal of genetics and genomics = Yi chuan xue bao
|October 12, 2024
概括
了解玉米中 (N) 代谢是提高N使用效率的关键. 特定的转录因子MADS26被确定为玉米植物中N的利用和耐受性至关重要.
科学领域:
- 植物生物学 植物生物学
- 遗传学 遗传学 是一个
- 农业科学 农业科学
背景情况:
- 对于植物的生长和发育至关重要.
- 在玉米等作物中提高利用效率 (NUE) 对可持续农业至关重要.
- 了解代谢的遗传调节对于增强NUE至关重要.
研究的目的:
- 研究两个玉米品种 (B73和Ki11) 的不同利用和耐受性背后的分子机制.
- 确定关键的调节因素,特别是转录因子 (TF),参与反应途径.
- 阐明MADS26在玉米代谢中的作用及其对差异性耐受性的贡献.
主要方法:
- 时间过程转录组分析,以比较基因表达模式,以应对酸盐变异.
- 构建和分析共同表达网络以确定监管模块和TFs.
- 通过过度表达MADS26的功能分析,以评估其对酸盐敏感性和酸盐利用率的影响.
主要成果:
- 在不同的酸盐条件下,在玉米B73和Ki11系之间观察到利用基因 (NUGs) 的明显表达模式.
- 在两条线路之间,在反应模块和TF监管网络中发现了显著的差异.
- 在B73网络中,MADS26是一种独特的TF,表现不同,并且在过度表达时发现可以增强酸盐敏感性和酸盐利用率.
结论:
- MADS26在玉米的代谢中起着重要作用,影响酸盐敏感性和酸盐利用率.
- 包括MADS26在内的确定的监管差异,为B73和Ki11之间观察到的独特耐受性提供了洞察力.
- 这些发现为提高使用效率和减少环境影响的玉米品种的育种提供了潜在的遗传目标.
相关概念视频
Transcription
146.7K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
146.7K
Key Elements for Plant Nutrition
18.7K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
18.7K
Transcription Attenuation in Prokaryotes
15.2K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.2K
Regulation of Expression at Multiple Steps
875
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
875
Combinatorial Gene Control
8.3K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.3K
General Transcription Factors
5.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.2K


