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Updated: Jun 12, 2025

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使用Beacon平台从免疫小鼠中分离抗原特异单克隆抗体的协议

Meilan Fu1, Xiangwei Wang1, Shubing Tang2

  • 1Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.

STAR protocols
|October 13, 2024
PubMed
概括

该Beacon平台能够有效地隔离抗原特异性血B细胞. 本协议详细说明了使用Beacon来隔离细胞,测序B细胞受体 (BCRs) 和表达抗体,适用于人类样本.

关键词:
抗体是对抗体的一种.细胞隔离细胞的隔离.蛋白质的表达和净化过程序列化是指测序的使用.一个单细胞单细胞.

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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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