液体扩展剂,保存温度和时间对绵羊精子质量的影响
E Díaz-Hernández1, P Bóveda1, C M Picazo1
1Grupo SaBio, IREC (UCLM-CSIC-JCCM), ETSIAMB, Albacete, Spain.
Reproduction in domestic animals = Zuchthygiene
|October 13, 2024
概括
羊的精子质量最好使用含有果糖和蛋黄的扩展剂来保持. 较高的温度 (23°C) 和延长存储超过24小时,会对精子运动性和体完整性产生负面影响.
科学领域:
- 动物繁殖 动物繁殖
- 精子保护 精子保护
- 低温生物学 低温生物学
背景情况:
- 优化精液扩展器对于在绵羊人工授精计划中保持精子生存能力至关重要.
- 了解温度和扩展器成分对精子质量的影响,对于成功保存精子至关重要.
研究的目的:
- 评估不同精液延长剂和保存温度对绵羊精子质量的有效性.
- 为了确定稀释羊精液的最佳储存时间.
主要方法:
- 羊精液使用Tris-酸盐-果糖基扩展剂与/或没有蛋黄,Tris-酸盐扩展剂与蛋黄以及商业扩展剂Biladyl®进行稀释.
- 稀释的精液在15°C和23°C保存了4,24,48和72小时.
- 评估了精子质量参数,包括运动性 (VCL) 和体完整性. 还测量了反应性氧物种 (ROS) 的产生.
主要成果:
- 含有果糖和蛋黄的扩展剂产生了优越的精液质量.
- 与15°C相比,较高的温度 (23°C) 增加了活性氧物种 (ROS) 的产生,降低了精子运动率 (VCL) 和体完整性.
- 在大多数评估参数中,在保存24小时后,精子质量显著下降.
结论:
- 推添加蛋黄的基于三酸和果糖的扩展剂,以保持最佳的羊精子保存.
- 较低的储存温度 (15°C) 和较短的保存时间 (≤24小时) 对于保持绵羊精子生存能力至关重要.
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