相关实验视频
Updated: Jun 10, 2025

08:26
Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites
Published on: February 14, 2020
6.9K
在Eimeria tenella外源性阶段的器官细胞基因组动态
Perryn S Kruth1, Taylor Lane1, John R Barta2
1University of Guelph, Guelph, ON, Canada.
Parasites & vectors
|October 13, 2024
概括
在Eimeria tenella卵囊中,在分泌过程中,细胞和线粒体DNA拷贝数发生显著的变化. 这项研究量化了这些细胞器基因组的动态,有助于寄生虫的识别.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 菌,如Eimeria tenella,是细胞内原生虫寄生虫,导致禽类重大疾病.
- 埃梅里亚菌卵囊必须分泌体,才能感染,这一过程涉及器官细胞基因组拷贝数的变化.
- 之前关于Eimeria spp.中细胞器基因组拷贝数量的报道. 已经显示出很大的差异.
研究的目的:
- 在Eimeria卵胞分泌过程中,与核基因组相比,量化核外基因组 (核核细胞和线粒体) 的相对丰度.
- 为了研究有机细胞基因组拷贝数在整个化过程和完成后的变化动态.
主要方法:
- 使用定量聚合酶链反应 (qPCR) 来测量基因组副本数量.
- 下一代测序支持qPCR进行准确的量化.
- 随着时间的推移,评估了相对于核基因组的器官细胞基因组丰度.
主要成果:
- 在64小时后,卵囊化达到93%,没有进一步的增加.
- 虫细胞DNA拷贝数在72小时内显示出显著的变化,然后在卵囊脱落时降至最大的60%.
- 线粒体DNA拷贝数在16小时和32小时之间呈现出两次显著的转变,通过脱落减少到最大的28%.
结论:
- 这项研究描述了在Eimeria spp.期间形细胞和线粒体基因组丰度的动态变化. 外源的阶段. 外源的阶段.
- 了解这些动态,可以了解寄生虫生物学.
- 外核目标可以用于更敏感,更准确的分子量化和Eimeria寄生虫的识别.
相关概念视频
Diversity of Protists I
1
Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
1
Export of Mitochondrial and Chloroplast Genes
3.6K
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
3.6K
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
12.0K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
12.0K
Genomic DNA in Prokaryotes
43.5K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.5K
Eukaryotic Evolution
32.7K
The endosymbiont theory is the most widely accepted theory of eukaryotic evolution; however, its progression is still somewhat debated. According to the nucleus-first hypothesis, the ancestral prokaryote first evolved a membrane to enclose DNA and form the nucleus. Conversely, the mitochondria-first hypothesis suggests that the nucleus was formed after endosymbiosis of mitochondria.
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
32.7K
Replication in Prokaryotes
24.6K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
24.6K

