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通过RNA测序揭开登革热感染的潜在基因生物标志物

Jeyanthi Suppiah¹, Saiful Safuan Md Sani², Safiah Sabrina Hassan³

¹Virology Unit, Infectious Disease Research Centre, Institute for Medical Research, National Institutes of Health, Ministry of Health Malaysia, 40170, Setia Alam, Selangor Darul Ehsan, Malaysia. jeyanthi@moh.gov.my.

|October 13, 2024

概括

这项研究使用RNA测序来识别登革热患者宿主基因表达变化. 发现几种基因是预测严重登革热的潜在生物标志物,有助于早期检测和诊断.

科学领域:

病毒学病毒学
基因组学就是基因组学.
免疫学免疫学免疫学

背景情况:

登革热病毒的复制取决于宿主细胞机制和免疫反应.
在登革热感染期间,宿主基因表达的变化尚不清楚.
识别宿主生物标志物可以改善登革热的预测和检测.

研究的目的:

使用RNA测序对登革热患者宿主转录组进行分析.
为了确定与登革热严重程度相关的差异表达基因 (DEGs).
发现潜在的宿主生物标志物用于登革热预测和诊断.

主要方法:

高通量RNA测序 (RNA-seq) 对16名登革热患者和10名健康对照进行了测序.
进行了差异基因表达分析以识别DEGs.
进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.

主要成果:

与对照组相比,在登革热患者中观察到显著的基因表达差异 (6466个具有警告迹象的登革热基因,3082个严重的登革热).
主要涉及的KEGG通路包括运输和代谢,信号转导,病毒性疾病和免疫系统.
在所有登革热患者中,几种基因 (例如IFI27,KCTD14,SDC1) 始终被上调.
确定了严重登革热预测的潜在生物标志物,包括TNFSF15,SERPINB2和CCL7.

结论:

通过RNA-seq对宿主转录组进行分析是发现登革热生物标志物的可行策略.
已识别的DEG和潜在的生物标志物可以提高登革热的诊断和预后能力.
这项研究为开发改进的登革热检测算法提供了基础.

关键词:

生物标志物生物标志物登革热病毒感染者血小板血小板血小板血小板血小板严重的登革热.转录组转录组就是一个转录组.

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