EMBL-MCF 2.0:一种LC-MS/MS方法和相应的库,用于使用低吸附度HILIC染色体的高可靠性向和非向代谢学
Svitlana Dekina1,2,3, Theodore Alexandrov4,5,6,7,8,9, Bernhard Drotleff10,11
1Metabolomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
Metabolomics : Official journal of the Metabolomic Society
|October 13, 2024
概括
一个拥有250多种代谢物的新库,EMBL-MCF 2.0,增强了代谢学中的代谢物识别. 本资源使用水友交互液体色谱 (HILIC) 和质谱学来获得高可靠性结果.
科学领域:
- 代谢学 代谢学 代谢学
- 分析化学 分析化学
- 生物化学 生化学
背景情况:
- 液体染色学-质谱学 (LC-MS) 在科学研究中至关重要.
- 代谢物识别 (MetID) 仍然是代谢学中的一个重大挑战.
- 高可信度的MetID需要将实验数据与真实的参考标准保持一致.
研究的目的:
- 开发EMBL-MCF 2.0 LC-MS/MS方法和公共图书馆.
- 促进内源性,极地代谢物的有针对性和非有针对性的代谢.
- 使用真实标准和先进染色学实现高可靠性的MetID.
主要方法:
- 使用水友互动液态色谱 (HILIC) 和低吸附LC硬件.
- 在轨道探测器Exploris 240上获得了高分辨率的双重质谱.
- 在QTRAP 6500+上获得了优化的多重反应监测 (MRM) 过渡.
主要成果:
- 生物相容的HILIC改善了同位素代谢物的分离,增强了选择性和灵敏性.
- 该库包含超过250种生物相关的代谢物.
- 该方法在小鼠血样本中得到了验证.
结论:
- 开发了EMBL-MCF 2.0库,用于敏感的代谢学和高可信度的MetID.
- 该图书馆可以在CC-BY 4.0许可证下以.msp格式自由访问.
- 为代谢学社区提供了宝贵的资源.
更多相关视频
11:00Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
22.4K
17:12Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS
Published on: December 20, 2010
15.6K
相关概念视频
01:21Mass Spectrometry: Complex Analysis
732
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
732
01:33Peptide Identification Using Tandem Mass Spectrometry
6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K