高通量测序确定了与伊拉克无花果马赛克疾病相关的多种无花果病毒和病毒
Nabeel Al-Kaeath1,2,3, Shrooq Zagier4, Osamah Alisawi4
1Institut National de la Recherche Agronomique de Tunisie, Université de Carthage, Laboratoire de Protection des Végétaux LR16INRAT04, Rue Hedi Karray, 1004 ElMenzah, Tunis, Tunisia.
The plant pathology journal
|October 14, 2024
概括
无花果植物的马赛克病是由多种病毒引起的,而不仅仅是无花果马赛克病毒. 高通量测序确定了无花果恶性病毒1,葡萄酒恶性病毒1和两个病毒,揭示了它们的遗传关系和在无花果基因组中的存在.
科学领域:
- 植物病毒学 植物病毒学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 马赛克病是无花果植物的一个重要问题,通常归因于无花果马赛克病毒.
- 然而,其他病毒病原体在无花果马赛克病的参与需要全面的识别和表征.
研究的目的:
- 用高通量测序研究无花果植物中马赛克病的病毒病因.
- 为了识别和描述症状无花果叶中存在的病毒和病毒.
- 为了对已识别的病毒序列进行遗传学分析.
主要方法:
- 从马赛克症状无花果叶的基因组DNA和总RNA的高通量测序 (Illumina平台).
- 生物信息分析用于识别病毒序列和组装基因组.
- 遗传学分析以确定已识别的病毒和病毒的进化关系.
主要成果:
- 识别无花果恶性病毒1 (FBV-1) 和葡萄恶性病毒1 (GBV-1),每个都有一个开放的读取框架,编码五个蛋白质域.
- 检测果外皮病毒 (CEVd) 和果果病毒 (ADFVd).
- 遗传学分析显示,FBV-1,GBV-1,CEVd和ADFVd分离物之间存在明显的地理关系.
- 发现一种低表达的内源性帕雷特病毒,caulimovirus-Fca1,集成到两个无花果系 ('Horaishi'和'Caprifig 6085') 的基因组中.
结论:
- 无花果的马赛克病与复杂的病毒群相关,包括badnaviruses和 viroids.
- 遗传学研究为这些病毒的起源和传播提供了洞察力.
- 内源性caulimovirus-Fca1代表了无花果基因组中的新发现,需要进一步调查.
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