在慢性病毒性肝炎中与炎症相关的长非编码RNA (lncRNAs) 与肝细胞癌相关的肝细胞癌
Burcin Pehlivanoglu1, Anil Aysal1, Cihan Agalar2
1Department of Molecular Pathology, Dokuz Eylul University, Graduate School of Health Sciences, IZMIR, TÜRKİYE.
Turk patoloji dergisi
|October 14, 2024
概括
与炎症相关的长非编码RNAs (lncRNAs) 在慢性病毒性肝炎 (CVH) 和相关的肝癌 (CVH-HCC) 中表现异常. DLEU2,SNHG16,LINC00662和XIST是CVH-HCC的潜在诊断标记物.
科学领域:
- 分子生物学分子生物学
- 肝病学 肝病学是一种肝病学.
- 在瘤学瘤学.
背景情况:
- 慢性病毒性肝炎 (CVH) 构成患肝细胞癌 (HCC) 的重大风险.
- 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在各种疾病中的作用,包括癌症.
- 了解CVH和CVH-HCC中与炎症相关的lncRNAs的表达模式对于识别潜在的生物标志物至关重要.
研究的目的:
- 研究慢性病毒性肝炎 (CVH) 和CVH相关肝细胞癌 (CVH-HCC) 中与炎症相关的lncRNAs的表达特征.
- 在CVH和CVH-HCC的背景下评估这些lncRNA的预后意义.
主要方法:
- 实时聚合酶连锁反应 (RT-PCR) 用于分析 84 个与炎症相关的 lncRNAs 在甲固定嵌 (FFPE) 组织样本中的表达.
- 表达水平在正常肝脏组织,肝硬化CVH副体,周肝硬化副体和CVH-HCC样本中进行了比较.
- 特定的lncRNAs (DLEU2,HOTAIR,LINC00635,LINC00662,RP11-549J18.1,SNHG16和XIST) 在更大的样本组中使用RT-PCR进一步验证.
主要成果:
- 与正常肝脏组织相比,在周周性肝硬化副体中观察到56个与炎症相关的lncRNAs的显着上调.
- 71个lncRNAs的表达在周围性肝硬化副体比肝硬化CVH副体显著更高.
- DLEU2和SNHG16在瘤和周围瘤肝硬化副血清中相对于肝硬化CVH副血清都被上调. 在CVH-HCC中,LINC00662显著更高. 在瘤和周围瘤样本中,XIST的表达增加,但没有统计学意义. 没有发现lncRNA表达和生存之间的显著关联.
结论:
- 与炎症相关的lncRNAs DLEU2,SNHG16,LINC00662和XIST被确定为CVH-HCC的潜在诊断生物标志物.
- 需要进一步的研究来确定它们作为CVH-HCC的预后标记物的有用性.
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