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相关概念视频

X-ray Diffraction of Biological Samples01:10

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X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Conservation biology is a scientific field that focuses on the preservation of biodiversity in order to protect ecosystems while meeting the needs of the human population. Humans require properly functioning ecosystems to maintain our supply of natural resources, including food, medicines, and building materials.
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Mass Spectrometry: Isotope Effect01:13

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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the difference between the molecular mass. Furthermore, the intensity of these signals is dependent on the...
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Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
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IBEX知识库:通过开放科学一起实现更多

Andrea J Radtke1, Ifeanyichukwu Anidi2, Leanne Arakkal3

  • 1Lymphocyte Biology Section and Center for Advanced Tissue Imaging, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

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概括

代漂白扩展多重Xity (IBEX) 是一种强大的成像技术,用于各种组织. IBEX知识库提供共享资源,以推进多重成像研究.

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科学领域:

  • 生物医学成像学 生物医学成像学
  • 分子生物学分子生物学
  • 细胞成像 细胞成像

背景情况:

  • 多复合成像技术使生物样本中的多个目标能够同时可视化.
  • 代漂白扩展多重Xity (IBEX) 是一种高度多重化的成像方法.
  • 不同的组织类型对成像协议构成挑战.

研究的目的:

  • 引入IBEX知识库作为多重成像的资源.
  • 支持使用或开发IBEX协议的研究人员.
  • 通过开放科学促进组织成像方面的创新.

主要方法:

  • 开发一个集中的IBEX知识库.
  • 针对IBEX的试剂和协议的汇编.
  • 坚持开放科学原则,以共享资源.

主要成果:

  • 现在可以获得一个关于IBEX成像的全面资源.
  • 该知识库可方便获取必要的试剂和协议.
  • 授权研究人员在多重组织成像中进行创新.

结论:

  • IBEX知识库促进了多重成像中的可访问性和可重复性.
  • 开放的资源共享加速了生物医学研究的进步.
  • 支持本资源的IBEX成像,为各种组织分析提供了一种多功能方法.