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死作为昆虫幼年激素生物合成的主要调节者

Takumi Kayukawa¹, Keisuke Nagamine¹, Tomohiro Inui¹

¹Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8634, Japan.

|October 14, 2024

概括

死亡声器 (Dri) 作为昆虫中青春激素 (JH) 生物合成基因的中央调节器. 这种转录因子控制着JH的产生,这对昆虫的变形和发育至关重要.

科学领域:

发展生物学发展生物学
昆虫内分泌学昆虫内分泌学
分子遗传学分子遗传学

背景情况:

青春激素 (JH) 对于维持幼虫特征和调节整体代谢昆虫的变形至关重要.
JH生物合成通过JH生物合成酶 (JHBEs) 在体 (CA) 中发生,具有协调的基因表达.
JH酸甲基转移酶 (JHAMT) 是JH合成的最后一步的关键酶,但其表达并不总是与其他JHBEs协调.

研究的目的:

确定负责昆虫CA中JHBE基因协调表达的中央调节器.
研究转录因子在调节JH生物合成和转化中的作用.
阐明控制CA特异性JHBE基因表达的上游监管机制,包括JHAMT.

主要方法:

在活性和非活性阶段对CA进行比较转录组分析,以确定潜在的调节者.
RNA干扰 (RNAi) 降低了候选转录因子的表达,包括死 (Dri).
评估转化表型和JHBE基因表达在敲击后,使用JH类型的救援实验.

主要成果:

三种转录因子显示出与JHBE基因相匹配的表达模式;死 (Dri) 被确定为关键调节者.
敲除Dri诱导了早发性变形,它被外源的JH模拟物所拯救,证实了Dri在JH调节中的作用.
驱动敲击降低了大多数CA特异性JHBE基因的表达,RNAi表明Dri在调节途径上游起作用.

结论:

死亡声器 (Dri) 作为CA特异性JHBE基因的中央上调器,包括JHAMT,在整体代谢昆虫中起作用.
通过控制JH生物合成,Dri在调节昆虫转化中发挥了新的作用,扩展了其在胚胎发育中的已知功能.
独立关闭JHAMT表明涉及一个未识别的压缩器下游或并行Dri.

关键词:

死亡的响应器响应器生物合成生物合成昆虫的发展昆虫的发展年轻人荷尔蒙的激素.变形转化是一种转化.

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