鼠标C57BL/6N亚菌株是弹性酶诱导的腹腔大动脉动脉瘤的一个可行的模型
Panpan Wei1,2, Kexin Li2, Meng Li1
1Department of Vascular Surgery, The Second Hospital of Tianjin Medical University, Tianjin, China.
Frontiers in cardiovascular medicine
|October 14, 2024
概括
C57BL/6N小鼠是使用猪胰腺弹性酶 (PPE) 输液治疗腹腔大动脉瘤 (AAA) 发展的合适模型. 这项研究证实了它的实用性,显示了与C57BL/6J亚株相比较的AAA诱导.
科学领域:
- 血管生物学 血管生物学
- 动物模型 动物模型
- 心血管研究研究心血管研究
背景情况:
- 腹腔大动脉瘤 (AAA) 是一个重要的心血管问题.
- 像C57BL/6J和C57BL/6N这样的小鼠亚株对某些AAA诱导方法具有不同的敏感性.
- 了解子菌株特异性反应对于准确的实验建模至关重要.
研究的目的:
- 评估C57BL/6N小鼠亚株对猪胰腺弹性酶 (PPE) 输液诱导的非剖析AAA的敏感性.
- 为了比较C57BL/6N小鼠的AAA发展与更容易受到影响的C57BL/6J亚株.
主要方法:
- 在C57BL/6J和C57BL/6N小鼠中,使用过渡性大动脉光道输液的PPE诱导了实验性非剖析的AAA.
- 在基线和输液后14天测量了腹腔大动脉直径.
- 对大动脉样本进行了组织病理学分析,以评估细胞透,弹性质降解和新血管化.
主要成果:
- 在PPE输液注入14天后,C57BL/6J和C57BL/6N小鼠都出现了显著的大动脉扩张 (>50%的直径增加),符合AAA模型标准.
- 在两个亚菌株中,PPE输液诱导了巨细胞和T细胞透,弹性质降解,光滑肌肉细胞枯竭和MMP-2/9表达升高.
- 与C57BL/6N小鼠相比,C57BL/6J小鼠在动脉瘤部位的B细胞招募和新血管化增加.
结论:
- 鼠标C57BL/6N亚菌株是一种可行且合适的模型,可通过弹性酶输液来建立实验性腹腔大动脉动脉瘤.
- 这一发现支持在AAA研究中使用C57BL/6N小鼠,特别是在研究非剖析AAA模型时.
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