相关实验视频

Updated: Jun 10, 2025

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

FOXN3-AS1:候选预后标记物和表观遗传标,在急性髓性白血病中具有免疫治疗影响

Fangfang Ge¹, Yulu Wang¹, Peng Chen¹

¹Department of Integrated Oncology, Center for Integrated Oncology (CIO), University Hospital of Bonn, Bonn, Germany.

Current medicinal chemistry

|October 14, 2024

概括

这项研究确定FOXN3-AS1作为急性髓性白血病 (AML) 预后的重要生物标志物. 高FOXN3-AS1表达表明AML患者的生存率较差,并且有针对性治疗的潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

新兴的高通量技术揭示了非编码RNA在血液恶性瘤中的作用.
综合的多omics数据集对于理解癌症病原体至关重要.

研究的目的:

研究FOXN3反感转录1 (FOXN3-AS1) 在急性髓性白血病 (AML) 中的作用.
评估FOXN3-AS1作为预后指标及其在AML中的潜在治疗影响.

主要方法:

临床AML样本上的定量聚合酶连锁反应 (qPCR).
在TCGA数据上使用卡普兰-梅尔和接收器操作特征曲线进行预测分析.
对FOXN3-AS1与基因突变和免疫功能的相关性分析.
对FOXN3-AS1/DNMT1相互作用的in silico预测和实验验证.
使用OncoPredict和GDSC数据库进行药物反应预测.

主要成果:

在AML患者中,FOXN3-AS1显著上调.
高FOXN3-AS1表达与不良预后和较差的免疫治疗反应相关.
FOXN3-AS1表达受到DNMT1抑制的影响.
某些化疗药物在高FOXN3-AS1表达率的AML患者中显示出良好的反应.

结论:

FOXN3-AS1作为AML存活的预后指标,具有潜在的免疫相关功能.
对于FOXN3-AS1/DNMT1的相互作用,需要进一步研究AML的新型免疫治疗策略.

关键词:

DNMT1.1. 在 DNMT1.1.这就是FOXN3N3的意思.在FOXN3-AS1中使用.急性骨髓性白血病 (AML) 是一种急性骨髓性白血病.反意义的成绩单的成绩单预后预测预测的预测.

更多相关视频

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...