微注射针的湿使用恒定的空气压力来反针的打开
1Insect Transformation Facility, The Institute for Bioscience and Biotechnology Research, University of Maryland; harrellr@umd.edu.
Journal of visualized experiments : JoVE
|October 14, 2024
概括
利的微注射针可以提高胚胎的存活率,用于基因组编辑. 湿提供了一致的针孔,这对于将CRISPR和转子子系统输入昆虫胚胎至关重要.
科学领域:
- 分子生物学分子生物学
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 微注射对于将CRISPR和转子子系统等基因组编辑工具输送到昆虫胚胎中至关重要.
- 与非形针相比,利的微注射针可以提高胚胎存活率.
- 针的一致性对于可复制的遗传物质的传递至关重要.
研究的目的:
- 为了强调尖的,形的微注射针对于昆虫基因组修改的重要性.
- 描述湿加工过程,以创建一致的微注射针.
- 解释湿如何允许控制针孔大小.
主要方法:
- 使用恒定的空气压力对微注射针进行湿.
- 监控气泡流量以确定针开口.
- 调整空气压力以达到平衡,并评估相对针孔大小.
主要成果:
- 湿 beveling 产生更一致的针孔比随机的尖端打破.
- 气泡的存在表明一个打开的针.
- 调整空气压力允许相对确定针孔大小,较低的压力表明更大的开口.
结论:
- 利的,形的微注射针对于成功修改昆虫胚胎的基因组至关重要.
- 湿是生产可控开口大小的一致针的可靠方法.
- 这种技术提高了提供基因组编辑组件的效率和可重复性.
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