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从低病毒载量临床标本中直接测序呼吸道病毒的基因组,使用目标捕获测序技术

Nobuhiro Takemae¹, Yumani Kuba¹, Kunihiro Oba²

¹Center for Emergency Preparedness and Response, National Institute of Infectious Diseases, Tokyo, Japan.

Microbiology spectrum

|October 14, 2024

概括

目标捕获测序显著提高了在低负载呼吸道样本中的病毒基因组检测,超过了SARS-CoV-2和流感A的元基因组测序. 这种方法有助于识别共感染和全长基因组.

科学领域:

病毒学病毒学
基因组学就是基因组学.
分子诊断学分子诊断学

背景情况:

基因组下一代测序 (mNGS) 由于病毒基因组副本数量较低,在临床样本中,特别是呼吸道病毒中,病毒载荷较低.
目标捕获测序 (TCS) 通过丰富特定的病毒基因组进行直接测序提供了一个解决方案.

研究的目的:

用一个全面的病毒探针面板来评估TCS的疗效,用于呼吸器样本.
评估TCS在检测低副本数量的SARS-CoV-2和流感A病毒基因组中的性能.

主要方法:

TCS被应用于来自SARS-CoV-2或A型流感患者的临床呼吸器样本.
病毒基因组丰富效率与mNGS进行了比较.
对低病毒载荷样本的检测灵敏度和基因组完整性进行了分析.

主要成果:

在低病毒载量下,TCS的读数比mNGS高180倍 (SARS-CoV-2) 和2000倍 (流感A).
在一些样本中,TCS检测到的病毒数量为<26个RNA拷贝/μL,对某些样本产生>70%的全长基因组.
TCS发现了与其他病毒的共同感染.

结论:

具有全面探针面板的TCS在检测和测序低负载呼吸道病毒,如SARS-CoV-2和流感A等方面非常有效.
这种方法显著提高了mNGS的敏感性,并有助于识别共感染.
在病毒感染的临床诊断和流行病学研究中,TCS具有前景.

关键词:

这就是SARS-CoV-2病毒.直接基因组测序是一种直接的基因组测序.甲型流感病毒是流感的一种病毒.这是下一代测序.呼吸道病毒的病毒目标捕获序列的测序

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