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相关概念视频

Condensins02:15

Condensins

3.4K
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
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Cohesins02:20

Cohesins

4.4K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
4.4K
Mitosis and Cytokinesis01:35

Mitosis and Cytokinesis

6.0K
In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
6.0K
Meiosis II01:57

Meiosis II

183.0K
Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each...
183.0K
Meiosis I01:49

Meiosis I

193.2K
Meiosis is a carefully orchestrated set of cell divisions, the goal of which—in humans—is to produce haploid sperm or eggs, each containing half the number of chromosomes present in somatic cells elsewhere in the body. Meiosis I is the first such division, and involves several key steps, among them: condensation of replicated chromosomes in diploid cells; the pairing of homologous chromosomes and their exchange of information; and finally, the separation of homologous chromosomes by...
193.2K
Forces Acting on Chromosomes02:11

Forces Acting on Chromosomes

3.3K
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis. 
Microtubules and motor proteins exert two types of forces on...
3.3K

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相关实验视频

Updated: Jun 10, 2025

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis
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A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

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线粒染色体的凝聚度有多大?

Hide A Konishi1, Hironori Funabiki1

  • 1Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY, USA.

The Journal of cell biology
|October 14, 2024
PubMed
概括

线性染色体在细胞分裂过程中实现了显著的紧缩. 新的研究显示,使用先进的显微镜技术,它们的核细胞度达到大约760μM.

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 线性染色体经历了重要的结构变化,以实现准确的细胞分裂.
  • 定量染色体体积和密度一直是细胞生物学中持续存在的挑战.

研究的目的:

  • 准确测量线性染色体的体积和核细胞度.
  • 为了提供新的见解染色体密集机制在线粒分裂期间.

主要方法:

  • 序列块面扫描电子显微镜 (SBF-SEM) 用于高分辨率成像.
  • 使用先进的成像和分析技术重建和量化染色体结构.

主要成果:

  • 发现线性染色体紧到高的核细胞度.
  • 这项研究报告了核细胞度约为760μM的凝聚的线粒染色体.

结论:

  • 这些发现提供了精确测量线粒体染色体紧缩的方法.
  • 这些定量数据有助于我们更好地理解细胞核内DNA包装的物理极限.

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Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
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