使用质谱成像技术,可视化了包装在奇多微针中的柏柏林通过皮肤传递的过程
Xiaoqing Cui1,2, Haoyuan Geng1,2, Huanying Guo1,2
1Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China.
Analytical and bioanalytical chemistry
|October 14, 2024
概括
基托桑微针阵列可以有效地通过皮肤传递柏柏林 (BR). 矩阵辅助激光吸附离子化质谱成像证实了皮肤层中的BR分布,显示了改善药物输送的潜力.
科学领域:
- 药理学和药物输送 药理学和药物输送
- 生物材料科学 生物材料科学
- 分析化学 分析化学
背景情况:
- 柏柏林 (BR) 是一种传统医学化合物,具有治疗潜力,但其口服生物利用性不佳.
- 通过皮肤递送药物提供了一种替代途径来克服口服限制.
- 微针阵列 (MNA) 是有希望的增强通过皮肤的药物透.
研究的目的:
- 开发和评估基多微针阵列 (CS MNAs) 用于通过皮肤输送柏柏林 (BR).
- 分析BR在异质皮肤组织中的空间分布和透.
- 证明MALDI-MSI的实用性,以评估通过皮肤传递的效率.
主要方法:
- 用柏柏林 (BR-CS MNA) 装载的奇多微针阵列的制备.
- 在皮肤模型中使用BR-CS MNA通过皮肤传递BR.
- 使用矩阵辅助激光吸附离子化质谱成像 (MALDI-MSI) 对皮肤组织中BR空间分布的分析.
- 利用内源性脂来区分皮肤层 (表皮和皮肤层).
主要成果:
- BR被成功加载并通过CS MNAs通过皮肤传递.
- MALDI-MSI成像显示BR的有效透到外皮和皮肤的皮肤层.
- 特定的内源性脂被确定为区分皮肤区域的标记物.
结论:
- 基托桑微针阵列为通过皮肤输送柏柏林提供了一个可行的平台.
- MALDI-MSI是一种强大的工具,用于评估通过皮肤递送药物的疗效和空间分布.
- 这种方法有望改善柏柏林和类似化合物的治疗应用.
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