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通过键相互作用和完全无标签的电化学生物传感器的微分脉冲电压测量来固定生物感应器

Adaris López-Marzo¹, Marta Mas-Torrent²

¹Institut de Ciència de Materials de Barcelona, ICMAB-CSIC, Campus UAB, 08193, Bellaterra, Spain.

Mikrochimica acta

|October 14, 2024

概括

用于检测乙型肝炎病毒 (HBV) 的无标签电化学生物传感器是使用简单的抗体不动化的方法开发的. 这种方法为医疗诊断提供了一个具有成本效益,效率和稳定的替代方案.

科学领域:

电化学电化学电化学
生物传感器技术技术
生物医学工程生物医学工程

背景情况:

无标签的电化学生物传感器在速度和成本上为点诊设备 (POCD) 提供了优势.
检测乙型肝炎病毒 (HBV) 对于临床诊断和环境监测至关重要.
现有的生物传感器通常依赖于标记的检测或电子转移介质,增加复杂性和成本.

研究的目的:

开发新的,无标签的电化学生物传感器,用于敏感和特定检测乙型肝炎病毒 (HBV).
研究不同抗体固定化策略 (结与共价结) 对生物传感器性能的影响.
将电化学阻抗光谱 (EIS) 和微分脉冲电压测量 (DPV) 作为HBV检测的读数技术进行比较.

主要方法:

修改黄金电极表面的氨酸 (CT) 或氨酸 (CS) 连接器.
通过键 (HB) 或共价键 (CB) 固定抗体 (Ab).
使用X射线光电子光谱 (XPS) 和原子力显微镜 (AFM) 进行表征;使用EIS和DPV进行电化学分析.

主要成果:

囊胺-结合 (CT-HB) 生物传感器与DPV相结合,显示出卓越的性能.
这种配置显示出更好的重复性,减少了血清矩阵干扰,以及与传统方法相比的检测/量化极限.
CT-HB生物传感器达到0.14 ng/mL的低检测极限,并保持了7天的稳定性.

结论:

通过键的抗体固定是一种简单,高效和低成本的替代 kovalent bond.
微分脉冲电压测量 (DPV) 比电化学阻抗光谱 (EIS) 提供了更快,更有效的读数.
开发的CT-HB生物传感器是快速,灵敏和稳定的HBV检测在护理场所设置的一个有希望的工具.

关键词:

微分脉冲电压测量微分脉冲电压测量黄金电极是黄金的电极.乙型肝炎病毒的感染.通过气结合,形成了气结合.没有标签的免疫传感器

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