高分辨率样本PET-CT与组织病理学联合注册的方法,以更好地了解放射性追踪器分布
Luna Maris1,2, Menekse Göker3, Jens M Debacker4,5,6
1Department of Electronics and Information Systems, Medical Image and Signal Processing, Ghent University, Ghent, Belgium. luna.maris@ugent.be.
EJNMMI physics
|October 14, 2024
概括
我们开发了一种方法,可以精确地将高分辨率的PET-CT图像与手术样本的组织病理学幻灯片对齐. 这种技术准确地绘制了组织中放射性追踪剂的吸收,有助于癌症研究.
科学领域:
- 医疗成像医学成像
- 病理学 病理学 病理学
- 放射化学 放射化学是指辐射化学.
背景情况:
- 在正子发射断层扫描 (PET) 中提升空间分辨率需要对射线追踪器分布的高分辨率理解.
- 实体样本PET,CT和组织病理学图像的同时注册对于详细分析至关重要.
研究的目的:
- 开发和验证一种方法,将高分辨率的微PET-CT图像与手术样本的整个幻灯片图像 (WSIs) 共同注册.
- 为了能够在亚毫米分辨率下分析与组织形态相关的放射性追踪器分布.
主要方法:
- 在使用[18F]氧葡萄糖 (FDG) 的乳腺癌 (BCa) 样本上应用了联合注册方法.
- 使用了一种图像采集方案,尽量减少组织变形 (微PET-CT,微CT,H&E染色WSI).
- 开发了一种由微CT指导的自动联合注册算法,使用微化估计空间误差.
主要成果:
- 对22个BCa标本的微PET-CT图像与WSI成功共同注册,平均误差为0.74 ± 0.17毫米.
- 证明 (前恶性) 组织通常比健康组织具有更高的标准化吸收值 (SUV).
- 在炎症组织和皮肤中观察到高吸收率,与预期一致.
结论:
- 开发了一种共同注册方法,其准确度与微型PET空间分辨率相当.
- 该方法适用于各种标本和放射性追踪器,以研究人类恶性瘤的分布,分辨率为亚毫米.
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