相关实验视频

Updated: Jun 10, 2025

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Published on: December 25, 2021

IntAct数据库用于访问IMEx的分子相互作用的上下文元数据

Kalpana Panneerselvam¹, Pablo Porras², Noemí Del-Toro^2,3

¹European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK.

Current protocols

|October 14, 2024

概括

国际分子交换联盟 (IMEx) 提供精选的分子相互作用数据,增强生物研究. 一个IMEx合作伙伴IntAct提供了一个庞大的,可搜索的蛋白质相互作用数据库,附有详细的实验证据.

科学领域:

分子生物学分子生物学
生物信息学是一种生物信息学.
系统生物学系统生物学

背景情况:

国际分子交换联盟 (IMEx) 是一个开放资源的伙伴关系,用于策划分子相互作用数据.
在这次合作中,IntAct,MINT,MatrixDB和DIP都是关键合作伙伴.
准确的分子相互作用数据对于理解细胞过程和疾病机制至关重要.

研究的目的:

描述IntAct分子相互作用数据库作为IMEx联盟中的一个关键资源.
突出IntAct.的综合数据策划,注释和可访问性功能.
展示IntAct的实用性,用于生成特定上下文的互动组,并促进协作研究.

主要方法:

来自科学文献的分子相互作用数据的聚合.
控制词汇和标准本体学的应用 (PSI MI) 用于数据标准化.
实施细粒度数据模型,捕捉实验细节和信心评分.
开发用户友好的搜索选项,交互式过器,以及用于数据探索的图形类型数据库.

主要成果:

IntAct数据库包含来自75,000个实验的120多万个二进制交互,遍及23000个出版物.
数据经过精心策划,重点关注实验背景和信心水平.
交互式工具和图形数据库可实现高级查询和网络分析.
该资源支持创建疾病或组织特定的互动体.

结论:

在IMEx框架内,IntAct是访问精选的分子相互作用数据的重要门户.
数据库的详细注释和高级搜索功能使研究人员能够探索复杂的生物网络.
持续的数据更新和协作功能确保IntAct仍然是科学界的宝贵资源.

关键词:

具有约束力的域名相互作用强度的相互作用强度.分子网络是分子网络.突变是发生在突变中的突变.蛋白质与蛋白质的相互作用

更多相关视频

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

A Data Integration Workflow to Identify Drug Combinations Targeting Synthetic Lethal Interactions

A Data Integration Workflow to Identify Drug Combinations Targeting Synthetic Lethal Interactions

Published on: May 27, 2021

相关概念视频

Protein-protein Interfaces

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...

Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...