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相关概念视频

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Updated: Jun 10, 2025

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
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人体脂肪细胞中的仓库特定的mRNA表达程序表明,通过不同的发育计划,可以通过生理专业化来进行生理专业化.

Heather J Clemons1,2, Daniel J Hogan1,2, Patrick O Brown1,2

  • 1Department of Biochemistry, Stanford University School of Medicine, Palo Alto, California, United States of America.

PloS one
|October 14, 2024
PubMed
概括

来自不同身体区域的人类脂肪细胞 (脂肪细胞) 是功能上不同的,专门的器官. 它们独特的基因表达模式表明了仓库特定的发育编程,而不仅仅是环境反应.

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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
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相关实验视频

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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
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科学领域:

  • 人类生理学 人类生理学
  • 发育生物学是发展生物学.
  • 分子遗传学 分子遗传学

背景情况:

  • 脂肪组织,包括不同的解剖储存,在很大程度上是未知的功能专业化.
  • 仓库特定特征 (内在编程与环境因素) 的起源仍然不清楚.

研究的目的:

  • 为了调查是否解剖学上不同的人类脂肪储存展示独特的功能特征.
  • 为了确定仓库特定的脂肪细胞特征是否来自内在的发育计划或微环境影响.

主要方法:

  • 用DNA微阵列来比较分离的人类脂肪细胞和培养脂肪干细胞中的mRNA表达.
  • 分析包括来自七个不同的解剖仓库的细胞,在体外分化之前和之后.

主要成果:

  • 来自不同仓库的脂肪细胞显示了不同的基因表达程序,反映了解剖学上相关的仓库.
  • 观察到基因表达特异性的基因表达差异,并在ex vivo分化的原生细胞中进行了回顾.
  • 发育转录因子显示出引人注目的仓库特定表达模式.

结论:

  • 来自各种解剖学库的脂肪细胞是功能上不同的专门器官.
  • 仓库特异性脂肪细胞专业化反映了不同的,决定性编程的发育路径.
  • 这些发现支持脂肪组织异质性的内在编程模型.