PavSPLs是甜桃生长,发育和应激反应的关键调节者
Xunju Liu1, Wanxia Sun1, Haobo Liu1
1Department of Plant Sciences, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai 200240, China.
概括
甜桃SQUAMOSA PROMOTER 结合蛋白样 (SPL) 基因对于生长和应激反应至关重要. 这项研究确定了16个SPL基因,揭示了它们在桃休眠恢复和生长调节中的作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 植物生理学 植物生理学
背景情况:
- 甲状腺促进体结合蛋白样 (SPL) 基因是重要的植物转录因子.
- 它们在甜桃 (Prunus avium) 发育和应激适应方面的特定功能在很大程度上仍未被描述.
研究的目的:
- 在甜桃基因组中识别和表征SPL基因.
- 研究这些基因在甜桃生长,休眠和对环境线索的反应中的作用.
主要方法:
- 16个SPL基因 (PavSPLs) 的全基因组识别和分离.
- 对基因结构,亚家族分类和 cis 调控元素的生物信息分析.
- 根据季节性变化和吉伯雷林 (GA) 治疗的表达形状.
- 在Arabidopsis thaliana中通过PavSPL14过度表达的功能分析.
- 使用酵母二混合 (Y2H) 进行蛋白与蛋白相互作用测定,用于PavSPL14和PavDWARF8.
主要成果:
- 发现了16个SPL基因,并将其分为5个子家族,12个基因被预测为miR156目标.
- 帕夫SPL基因具有与光,压力和激素信号通路相关的cis元素.
- 季节性表达模式表明,PavSPLs参与了甜桃的休眠后恢复.
- GA治疗抑制了PavSPL表达,表明参与GA介导的途径.
- 在Arabidopsis中PavSPL14的过度表达促进了早期的开花和增强植物生长.
- PavSPL14与DELLA蛋白PavDWARF8相互作用,这表明增长的共同调节.
结论:
- 鉴定到的PavSPL基因是甜桃生长,发育和非生物应激反应的潜在调节者.
- PavSPL14在花期和植物高度方面发挥作用,与DELLA蛋白相互作用以调节生长.
- 这项研究为了解和操纵SPL基因功能在甜桃育种和种植中的基础.
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