来自Agaricus bisporus的具有三重螺旋结构的多糖:特征和抗结肠癌活性
Kangzhen Dong1, Junhui Wang1, Fangyuan Tang2
1Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230601, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China; The Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, Hefei 230009, China.
International journal of biological macromolecules
|October 14, 2024
概括
从Agaricus bisporus中分离出的新型三螺旋多糖体WAAP-2显示出显著的抗结肠癌特性. 它有效地诱导细胞亡,并抑制癌细胞迁移和入侵,为新的治疗应用铺平了道路.
科学领域:
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
- 癌症研究 癌症研究
背景情况:
- Agaricus bisporus是一种常见的食用,具有潜在的药用特性.
- 众所周知,多糖具有各种生物活性,包括抗癌作用.
- 来自自然来源的新生物活性化合物的分离和表征对于药物发现至关重要.
研究的目的:
- 从Agaricus bisporus中分离和表征一种新型多糖体 (WAAP-2).
- 为了研究WAAP-2的抗结肠癌活性.
- 阐明WAAP-2在结直肠癌细胞中的作用机制.
主要方法:
- 从Agaricus bisporus中分离和净化WAAP-2多糖体.
- 对WAAP-2进行物理化学和结构分析 (包括初级和高级结构确定).
- 使用HT-29细胞进行体外抗结肠癌试验,包括细胞周期分析,细胞亡试验和迁移/入侵试验.
主要成果:
- 一种具有三螺旋结构的121kDa多糖体WAAP-2被成功分离出来.
- WAAP-2包括曼诺糖,葡萄糖和银糖,具有确定的单糖链接位置.
- 在HT-29细胞中,WAAP-2诱导了细胞亡,通过在S阶段阻止细胞循环,对Caspase-3和Bax进行上调,并对Bcl-2进行下调.
- WAAP-2通过调节E-cadherin和Vimentin的表达来抑制HT-29细胞的迁移和入侵,影响表皮-介质细胞过渡.
结论:
- WAAP-2是一种来自Agaricus bisporus的新型三螺旋多糖,具有显著的抗结肠癌活性.
- WAAP-2通过诱导细胞亡和抑制细胞迁移和入侵来发挥其抗癌作用.
- WAAP-2显示出作为结直肠癌治疗的低毒性治疗剂的潜力.
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