针对线粒体的纳米颗粒稳定了Sargassum fusiforme多糖体增加反应性氧物种介导的抗瘤活性
Yanzhe Chen1, Feifei Zhu1, Jieying Ou1
1College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, China; Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang, China; Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang, China; Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang, China; Guangdong Provincial Modern Agricultural Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Zhanjiang, China.
International journal of biological macromolecules
|October 14, 2024
概括
聚糖稳定纳米颗粒 (SFPS-Tw-SeNPs) 显示出显著的抗肺癌作用. 这些纳米颗粒诱导了亡并抑制了体内瘤的生长,为非小细胞肺癌治疗提供了潜力.
科学领域:
- 纳米技术纳米技术
- 生物医学工程 生物医学工程
- 药理学 药理学是指药理学的学科.
背景情况:
- 肺癌仍然是全球癌症相关死亡的主要原因.
- 新的治疗策略对于改善治疗结果至关重要.
- 纳米粒子 (SeNPs) 已经证明了潜在的抗癌特性.
研究的目的:
- 在体外和体内研究Sargassum fusiforme多糖稳定纳米粒子 (SFPS-Tw-SeNPs) 的抗肺癌疗效.
- 阐明SFPS-Tw-SeNPs诱导的抗癌作用的潜在机制.
主要方法:
- 在A549肺癌细胞上进行了细胞活力测定,亡测定 (AO/EB染色),反应性氧物种 (ROS) 检测和细胞周期分析.
- 评估了线粒体膜潜力,Bax/Bcl-2比率,以及与亡相关的蛋白质 (Cleaved-Caspase 3,9,PARP,Cytochrome C) 的表达.
- 评估了PI3K/AKT信号通路,细胞周期调节剂 (Cyclin-A,CDK2,P21) 和A549异种移植小鼠模型中的瘤生长.
主要成果:
- SFPS-Tw-SeNPs通过增加ROS产量和破坏细胞膜,有效地抑制了A549细胞增殖和诱导细胞亡.
- 将SFPS-Tw-SeNPs内化到细胞中导致线粒体功能障碍,膜潜能降低,并通过酶激活和细胞染色体c释放进行亡.
- SFPS-Tw-SeNPs通过调节PI3K/AKT通路和调节细胞循环蛋白 (降低Cyclin-A,CDK2;上调P21) 来阻止G1阶段的细胞.
- 在体内研究表明,显著抑制A549瘤异种移植的生长,没有显著的不良影响动物健康.
结论:
- SFPS-Tw-SeNPs通过多种机制表现出强大的抗肺癌活性,包括诱导亡和细胞循环停止.
- 这些纳米粒子在体内表现出良好的安全性,表明它们作为治疗非小细胞肺癌的治疗剂的潜力.
- SFPS-Tw-SeNPs代表了开发用于预防和治疗肺癌的新药的有希望的候选人.
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