相关实验视频
Updated: Jun 10, 2025

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
Published on: January 31, 2025
AKT/mTOR介导的自信号与TCDD诱导的口腔裂有关
Yaxin Zhang1, Dongliang Xu2, Shuaixing Song3
1Center for Clinical Single-Cell Biomedicine, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, Henan 450003, China; Department of Nutrition and Food Hygiene, Public Health College, Zhengzhou University, Henan 450001, China.
对2,3,7,8-四二二氧化 (TCDD) 的环境暴露增加了口腔裂 (CP) 的风险. 通过AKT/mTOR信号传递,TCDD抑制了小鼠胚胎 palatal mesenchymal 细胞的增殖和自,从而导致CP.
科学领域:
- 发育生物学是发展生物学.
- 环境毒理学环境毒理学
- 分子生物学分子生物学
背景情况:
- 在子宫内暴露于环境污染物2,3,7,8-四二二二氧化物 (TCDD),与 palatal cleft (CP) 形成的增加率有关.
- 在TCDD诱导的CP背后的精确分子机制仍然不完全理解.
研究的目的:
- 通过多omics方法阐明TCDD诱导的口腔裂的病因基础.
- 为了研究TCDD对细胞增殖,自和 palatal mesenchymal 细胞中信号通路的影响.
主要方法:
- 在怀孕14.5天 (GD14.5) 的胚胎上进行了多组分析 (代谢和转录).
- 评估了小鼠胚胎 palatal mesenchymal (MEPM) 细胞的增殖和自活动.
- 电子显微镜和西部免疫血清被用来评估与自和AKT/mTOR信号传递相关的蛋白质水平.
主要成果:
- TCDD诱导的CP形成与,MAPK,PI3K-Akt和mTOR信号通路的失调有关.
- 暴露于TCDD抑制了MEPM细胞的增殖和自活动.
- 在暴露于TCDD的细胞中观察到AKT/mTOR信号的同时激活,这与多omics数据一致.
结论:
- 通过激活AKT/mTOR信号,TCDD暴露会通过抑制增殖和自来破坏口腔细胞平衡.
- 这种细胞过程的破坏有助于在子宫内暴露于TCDD后的口腔裂的发展.
更多相关视频
19:44Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
Published on: May 30, 2012
07:13Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
Published on: February 13, 2021
相关概念视频
PI3K/mTOR/AKT Signaling Pathway
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Role Of Notch Signalling In Intestinal Stem Cell Renewal
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Autophagy
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Delivery Pathways to the Lysosome
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Autophagic Cell Death
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...