在黑综合征的鱼中,CBS/CSE介导的H2S生产通过Toll通路诱导AMPs的表达
Ziyue Zhu1, Xingkong Ma2, Xiaohan Liu1
1Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, College of Marine Science and Engineering, Nanjing Normal University, Nanjing, 210023, Jiangsu Province, China.
Fish & shellfish immunology
|October 14, 2024
概括
在Eriocheir sinensis中,黑症候群涉及硫化 (H2S) 和抗菌 (AMP) 的增加. 由CBS/CSE监管的H2S通过通道调节AMP,提供疾病管理见解.
科学领域:
- 水产养殖免疫学 水产养殖免疫学
- 甲动物病理学 甲动物病理学
- 分子生物学分子生物学
背景情况:
- 黑综合征 (BGS) 对Eriocheir sinensis水产养殖构成重大威胁.
- 抗微生物 (AMP) 是类天生的免疫系统的重要组成部分.
- 了解BGS的发病因子可以为疾病预防和治疗策略提供信息.
研究的目的:
- 调查受 BGS.影响的 Eriocheir sinensis 中的天生的免疫反应.
- 阐明特定免疫基因和硫化 (H2S) 在BGS中的作用.
- 在BGS中确定H2S,收费通道组件和AMP之间的监管关系.
主要方法:
- 对抗脂多糖因子 (ALFs),Toll受体3 (Toll3),Pelle激酶 (Pelle),cystathionine β-synthase (CBS) 和cystathionine γ-lyase (CSE) 的基因表达分析.
- 在中测量H2S含量.
- 实验性操纵包括H2S暴露和基因敲除 (CBS,CSE).
主要成果:
- 在E. sinensis中,EsALF7,EsALF-L,EsToll3和EsPelle的表达与BGS进行了上调.
- 在受影响的上观察到增加的H2S含量和调高的EsCBS和EsCSE表达.
- 暴露于H2S上调了EsToll3,EsPelle,EsALF7和EsALF-L;击败EsCBS/EsCSE降低了它们的表达.
结论:
- 由CBS/CSE调节的H2S的升级促进了ALFs通过BGS的E. sinensis的Toll路径的表达.
- 这项研究揭示了一种新的分子机制,它将H2S代谢与的先天免疫力联系起来.
- 这些发现为开发针对水产养殖中BGS的有针对性的干预措施提供了基础.
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