Buddlejasaponin IVb通过Nrf2/GPX4通路和肠道微生物群调节缓解DSS诱导的性结肠炎
Xiaodong Wu1, Linxian Zhao1, Zhihai Yu2
1Department of General Surgery, The Second Hospital of Jilin University, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun 130000, China.
布德尔杰沙宁IVb (BJP-IVb) 通过减少炎症和修复肠道屏障,有效治疗性结肠炎 (UC). 这种天然化合物激活Nrf2/HO-1通路,为结肠炎提供了一种新的治疗方法.
科学领域:
- 药理学 药理学是指药理学的学科.
- 胃肠病学 胃肠病学
- 自然产品 化学 化学
背景情况:
- 性结肠炎 (UC) 是一种慢性炎症性肠病,治疗选择有限.
- Buddlejasaponin IVb (BJP-IVb) 是一种来自*Pleurotus ostreatus*的素,具有生物活性特性,但其对UC的疗效尚不清楚.
- 了解UC中BJP-IVb的分子机制对于开发新疗法至关重要.
研究的目的:
- 在性结肠炎 (UC) 的小鼠模型中研究Buddlejasaponin IVb (BJP-IVb) 的治疗潜力和潜在机制.
- 阐明Nrf2/HO-1抗氧化途径和铁死在BJP-IVb对大肠炎的保护作用中的作用.
主要方法:
- 在小鼠中诱导大肠炎使用硫酸 (DSS).
- 管理BJP-IVb和临床和组织学参数的评估.
- 对Nrf2/HO-1通路激活,铁亡标记物和肠道微生物群组成的分析.
- 使用Nrf2-Knockout (KO) 小鼠和ML385 (Nrf2抑制剂) 的实验.
主要成果:
- BJP-IVb显著改善了DSS诱导的UC症状,包括体重减轻,疾病活动和结肠损伤.
- BJP-IVb修复了肠道屏障功能,抑制了炎症,并激活了Nrf2/HO-1抗氧化途径.
- 通过Nrf2/GPX4轴,BJP-IVb抑制了铁亡,改善了肠道微生物群的失活性.
- 根据对ML385和Nrf2-KO小鼠的实验,BJP-IVb的保护作用取决于Nrf2激活.
结论:
- 布德尔杰萨宁IVb (BJP-IVb) 显示出对性结肠炎 (UC) 的显著治疗和保护功效.
- BJP-IVb通过激活Nrf2/HO-1通路,抑制铁亡,调节肠道微生物群来发挥其保护作用.
- 这些发现强调了BJP-IVb作为UC的有希望的天然治疗剂,并建议针对肠道微生物群代谢的新策略.
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