通过ML增强的过氧酶体容量使多酶途径的细分成为可能
Jordan J Baker1,2,3, Jie Shi3,4, Shangying Wang3,4,5
1Department of Bioengineering, University of California, Berkeley, CA, USA.
Nature chemical biology
|October 14, 2024
概括
研究人员在Saccharomyces cerevisiae中改造过氧体,以提高代谢酶的功能. 这种增强的细分增加了80%,证明了一种新的代谢工程策略.
科学领域:
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
- 酵母生物技术 酵母生物技术
背景情况:
- 在Saccharomyces cerevisiae中重新定位的器官模仿了植物组织的代谢细分.
- 过氧体可以为工程代谢分隔异质酶,但通常被抑制.
- 低氧体功能能力限制了工程代谢途径的效率.
研究的目的:
- 为增强功能能力而设计过氧体,以支持复杂的代谢途径.
- 将多达八种代谢酶分隔在过氧体内,以改善生物合成标位.
- 利用机器学习来识别基因标以提高氧体功能.
主要方法:
- 在Saccharomyces cerevisiae中工程化过氧体,以增强异质酶表达.
- 实施了机器学习管道,以识别过度表达的目标,以改善过氧酶体功能.
- 量化过氧体的功能能力和杰拉尼奥产量.
主要成果:
- 与野生类型菌株相比,氧体功能能力增加了137%.
- 在工程化过氧体内成功分离了多达八种代谢酶.
- 证明基拉尼生物合成标位增加了80%,达到9.5g/L.
结论:
- 工程过氧体功能能力是提高代谢途径效率的可行策略.
- 机器学习有效地确定了改进基于器官的代谢工程的目标.
- 在酵母中增强的细分显著提高了价值的化合物的生产,如geraniol.
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