季节性环境波动改变了牛肉母牛卵细胞和粒粉细胞的转录组动态
Kamryn Joyce1, Ahmed Gad2,3, Nico G Menjivar2
1North Florida Research and Education Center, University of Florida, Marianna, FL, 32446, USA.
Journal of ovarian research
|October 14, 2024
概括
季节性热应激显著改变牛肉卵细胞和粒粉细胞的基因表达,影响生育能力. 了解这些细胞反应对于改善在气候变化中的生殖成功至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 环境生理学环境生理学
- 基因组学就是基因组学.
背景情况:
- 牛的生育能力下降越来越多地与环境温度升高有关.
- 了解细胞对热应激的反应对于解决生育问题至关重要.
- 季节性温度波动为研究热应激效应提供了一个自然模型.
研究的目的:
- 为了研究卵细胞和粒粉细胞对肉牛季节性温度变化的差异性基因表达反应.
- 为了将环境条件与热应激的生理标志物相关联.
主要方法:
- 在夏季和冬季通过卵子采集 (OPU) 从肉牛中收集积卵细胞复合体和粒粉细胞.
- 在聚合卵细胞和粒粉细胞上进行RNA测序,以分析基因表达特征.
- 测量了直肠温度,白细胞热冲击蛋白 (HSP70和HSP90) 的转录丰度,以及血皮质醇水平.
主要成果:
- 与冬季相比,夏季条件 (较高的温度和THI) 导致直肠温度和白细胞HSP70表达增加.
- 在夏季,在卵细胞和粒状细胞中观察到显著的基因表达差异,受影响的特定途径 (例如,ECM受体相互作用,卵细胞半转化,细胞粘附,新陈代谢).
- 季节间的HSP90表达或血皮质醇水平没有显著变化.
结论:
- 季节性温度变化对牛卵细胞和粒状细胞的转录组景观产生深远的影响.
- 这些分子变化可能会导致生育结果的季节性变化.
- 研究结果强调,需要制定策略来减轻热应激对牛繁殖的影响.
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