长期COVID增加了MMP-9和由SARS-CoV-2的微质释放的Spike蛋白质
Duraisamy Kempuraj1, Irene Tsilioni2, Kristina K Aenlle1,3
1Institute for Neuro-Immune Medicine (INIM), Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Ft. Lauderdale, FL, 33328, United States of America.
Translational neuroscience
|October 15, 2024
概括
矩阵金属蛋白酶-9 (MMP-9) 在长期COVID患者中升高,并导致神经精神症状. 类黄类药物,如黄素,显示出抑制MMP-9释放的潜力,为长期COVID治疗提供了治疗点.
科学领域:
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
背景情况:
- 长期的COVID带来了重大挑战,原因不明的慢性神经精神病症状.
- 矩阵金属蛋白酶-9 (MMP-9) 涉及破坏神经元连接,在长期COVID患者中发现的水平更高.
研究的目的:
- 调查MMP-9在长期COVID病变发生过程中的作用.
- 为了确定SARS-CoV-2尖端蛋白是否刺激MMP-9从微质细胞释放.
- 评估特定黄类药物对MMP-9释放的抑制作用.
主要方法:
- 从长期COVID患者和健康对照组的血清中量化了MMP-9水平,使用酶相关免疫吸收试验 (ELISA).
- 人类微质细胞系被SARS-CoV-2尖端蛋白,脂多糖 (LPS) 或神经素 (NT) 刺激.
- 评估了黄素和甲卢特预处理对斯派克蛋白诱导的MMP-9释放的影响.
主要成果:
- 与对照组相比,长期COVID患者的血清MMP-9水平显著更高.
- 当被LPS,NT或Spike蛋白刺激时,微细胞系释放出大量的MMP-9.
- 丁醇和甲卢特的预处理明显抑制了尖端蛋白诱导的MMP-9释放.
结论:
- 从Spike蛋白刺激的微质中增加的MMP-9可能会导致长期COVID的神经精神症状.
- MMP-9为长期COVID提供了潜在的治疗点.
- 黄类药物,如黄素,通过抑制MMP-9释放,显示出作为长期COVID治疗的潜力.
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