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光敏剂-ATP结合盒 (ABC) 载体相互作用的查体外
Shruti Vig1, Payal Srivastava1,2, Idrisa Rahman1,3,2
1Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
七种光敏剂被测试以使用ATP结合带 (ABC) 运输器进行运输. 孟加拉,红波芬和波芬衍生物是ABC转运器的基质,可能导致光动力学疗法耐药性.
科学领域:
- 生物化学 生化学
- 药理学 药理学是指药理学的学科.
- 在瘤学瘤学.
背景情况:
- ATP结合盒 (ABC) 载体调解癌细胞的药物外流,影响治疗疗效.
- 了解光敏剂与ABC传送器的相互作用对于优化光动力学疗法 (PDT) 至关重要.
研究的目的:
- 为了研究七种临床上使用的光敏感剂对ABC转运器介导的流动的敏感性.
- 为了确定特定的ABC载体 (P-gp,BCRP,MRP1) 是否影响光敏感剂细胞内积累和PDT疗效.
主要方法:
- 使用了过度表达P-gp (ABCB1),BCRP (ABCG2) 或MRP1 (ABCC1) 的人类乳腺癌细胞系.
- 通过流式细胞计量评估光敏剂细胞内水平,使用或不使用ABC输送抑制剂.
- 评估PDT后的细胞活力,以将输送活性与治疗耐药性相关联.
主要成果:
- 孟加拉,松衍生物 (BPD) 和红松被确定为P-gp,BCRP和/或MRP1.1的基质.
- 热波芬,塔拉波芬,甲基蓝和氨酸绿不是这些ABC载体的基质.
- 过度表达的ABC载体导致PDT耐药性细胞治疗孟加拉,redaporfin,和BPD.
结论:
- 热波芬,塔拉波芬,甲基蓝和氨酸绿不是P-gp,BCRP或MRP1.1的基质.
- 孟加拉,redaporfin和BPD与ABC转运体相互作用,影响它们的细胞内积累和PDT疗效.
- 亚贝克载体基质状态是细胞对特定光敏感剂的PDT抵抗的潜在机制.
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