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在五种乳酸细菌物种中比较等离子体治愈效率
Chan-Hyeok Park1,2, Haneol Yang1, Seunghyun Kim1,2
1Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56212, Republic of Korea.
Journal of microbiology and biotechnology
|October 15, 2024
概括
这项研究探讨了乳酸细菌中的等离子体治愈,以满足益生菌批准标准. 乙化和新生物素显示出可变的等离子体去除效率,受等离子体大小和菌株的影响.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 益生菌研究 益生菌研究
背景情况:
- 益生菌批准的严格规定需要删除抗生素耐药性基因 (ARG) 和毒性因子.
- 等离子体治愈是消除ARG和毒性基因的关键方法,以满足益生菌的监管要求.
研究的目的:
- 在五种含有等离子体的乳酸细菌菌株中研究使用乙基化物,色和新生物素的等离子体固化效率.
- 检查化学剂,度,持续时间和等离子体特性对硬化效率的影响.
主要方法:
- 使用不同度和治疗持续时间的乙基化物,酸和新生物素诱导了等离子治愈.
- 五种带有等离子体的乳酸细菌菌株接受了这些治疗.
- 评估了治愈效率,并对治愈的菌株进行了基因组分析.
主要成果:
- 塑固化效率在各种菌株和药剂之间有很大的差异,从1%到44%不等.
- 较大的等离子体和多个等离子体的菌株表现出较低的固化效率,需要更长的治疗时间.
- 在治愈后观察到一些菌株的抗生素敏感性发生变化,但与ARG损失没有一致的联系;基因组分析显示最小的突变.
结论:
- 等离子体治愈是一种可行的,但对菌株和等离子体依赖的益生菌发展策略.
- 优化固化条件至关重要,特别是对于具有多个或大型等离子体的菌株.
- 需要进一步的研究来证实等离子体损失和抗生素耐药性表型变化之间的直接联系.
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