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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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相关实验视频

Updated: Jun 10, 2025

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

使用Prime编辑对小鼠进行基因修改.

Amr R Salem¹, Xiaoling Xie², Susan H Griffin¹

¹Vascular Biology Center, Medical College of Georgia at Augusta University, Augusta, Georgia.

Current protocols

|October 15, 2024

概括

总编辑提供了一种简化的方法来创建转基因小鼠,克服了漫长和复杂的传统方法. 这种先进的基因编辑技术简化了这一过程,提高了小鼠基因改变的成功率.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?基因编辑基因编辑引导RNA指导RNA的指导RNA是指导RNA.微注射微注射的方法这里是鼠标鼠标鼠标鼠标鼠标鼠标.主编辑主要编辑.

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Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

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Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

相关实验视频

Last Updated: Jun 10, 2025

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
动物模型动物模型

背景情况:

传统的小鼠基因修改是劳动密集型的,需要广泛的步骤,如构造设计,干细胞操纵,和木乃伊繁殖.
这些传统方法耗时 (通常超过一年),昂贵,并且没有保证成功,需要专门的设施或供应商.
由RNA引导的CRISPR系统的出现简化了基因编辑,使得在小鼠和其他具有挑战性的动物模型中进行高效的修改成为可能.

研究的目的:

提出一种简化方案,用于生成转基因小鼠.
引入主要编辑平台作为鼠标基因工程的简化方法.
详细介绍了一种简化方法,用于在小鼠中创建基因修改.

主要方法:

设计,克隆和合成工程原始编辑指导RNA (pegRNA).
微注入原始编辑 (PE2) 组件到小鼠囊中.
创始老鼠的基因定型和随后的繁殖用于生殖系传播.

主要成果:

主要编辑平台可高效地在小鼠中产生基因修改 (替代,插入,删除).
这种方法简化了创建转基因小鼠的复杂过程.
通过描述的协议,成功实现了生殖系传播.

结论:

主编辑提供了一个显著简化和高效的替代传统方法产生转基因小鼠.
该协议使得小鼠的基因编辑成功率高,减少了项目的时间表和复杂性.
描述的方法使基因编辑小鼠模型的生成民主化.