染色体可访问性和基因表达在阿拉比多普西斯 (Arabidopsis arenosa) 的新型和进化的自立多体之间有所不同
Thanvi Srikant1, Adrián Gonzalo1, Kirsten Bomblies1
1Department of Biology, Institute of Molecular Plant Biology, ETH Zürich, Zürich, Switzerland.
Molecular biology and evolution
|October 15, 2024
概括
全基因组重复 (WGD) 导致多重体的初始表观遗传变化,但大多数基因表达干扰在进化多重体中会恢复. 在WGD上染色体可访问性变化与可转移元素有关,而不是基因表达.
科学领域:
- 植物生物学 植物生物学
- 基因组学就是基因组学.
- 进化生物学是进化的生物学.
背景情况:
- 全基因组复制 (WGD) 是真核生物基因组进化的主要驱动因素,影响复杂性,新性和适应性.
- 多倍体在WGD后经常表现出静态表观遗传和转录变化,但这些变化在进化的多倍体中的持续性仍然不清楚.
研究的目的:
- 为了研究在Arabidopsis arenosa中全基因组重复 (WGD) 后表观遗传和转录变化的持久性.
- 为了比较染色体可访问性和基因表达模式,在A. arenosa. 的二倍体,新四倍体和进化四倍体细胞型之间进行比较.
主要方法:
- 使用ATAC-seq来评估局部染色体的可访问性.
- 使用RNA-seq分析匹配的叶子和花样本中的基因表达.
- 比较了双胞胎,人工生成的新四胞胎和自然发生的进化四胞胎A. arenosa. 的基因组数据.
主要成果:
- 鉴定了超过8000个差异可访问的染色体区域,主要是组织特异的,在WGD上产生的.
- 发现只有很小一部分染色质可访问性变化与改变的基因表达有关.
- 观察到,细胞型之间的可访问性变化与附近可转移元素的数量密切相关.
- 指出,在基因组复制时,大多数差异性表达的基因在进化四体中恢复到接近双体的水平.
结论:
- 在进化过程中,对WGD的初始表观基因和转录反应并不总是保持在自身多重体中.
- 在WGD之后的染色质可访问性和基因表达变化不能直接相互预测.
- 与新形成的多倍体相比,进化的多倍体表现出明显的表观遗传和转录特征.
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