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肠道微生物的碳和硫代谢支持沙门氏菌感染

Ikaia Leleiwi^1,2, Katherine Kokkinias^2,3, Yongseok Kim⁴

¹Department of Cell and Molecular Biology, Colorado State University, Plant Sciences Bldg. 307 University Ave, Fort Collins, CO 80523, United States.

The ISME journal

|October 15, 2024

概括

沙门氏菌Typhimurium感染改变了肠道微生物群,揭示了关键的微生物相互作用和代谢途径. 了解这些变化为治疗沙门氏菌感染提供了新的目标.

科学领域:

微生物生态学微生物生态学
肠道微生物组研究研究
传染病的发病因子传染病的发病因子

背景情况:

沙门氏菌 (Salmonella enterica serovar Typhimurium) 是一个严重的公共卫生威胁.
在沙门氏菌感染期间,人们对肠道微生物群生态的了解很少.
微生物组介导的相互作用影响沙门氏菌的殖民和感染.

研究的目的:

在沙门氏菌感染期间调查肠道微生物群的微生物生态.
了解沙门氏菌如何重塑肠道微生物组.
为了确定沙门氏菌-宿主-微生物群的相互作用.

主要方法:

从感染和未感染小鼠的便微生物群体的多组分析 (metatranscriptomics, metabolomics).
基因组重建,将多个omics数据与特定的微生物联系起来.
对微生物基因表达和代谢物概况的分析.

主要成果:

沙门氏菌感染减少了肠道微生物的多样性,但增加了像Luxibacter和Ligilactobacillus这样的特定种群的转录映射.
氧化耐受机制在沙门氏菌引起的炎症期间对微生物生存至关重要.
肠道微生物群将有机硫转化为无机硫化合物 (thiosulfate,tetrathionate),支持沙门氏菌的呼吸.
乳酸是炎症肠道微生物群中产生的和消耗的关键代谢物.

结论:

多组组学提供了在沙门氏菌感染期间肠道微生物群代谢的应变解析图.
特定的微生物种群和代谢途径在感染期间被动态调节.
已确定的代谢途径,特别是硫代谢,代表了针对沙门氏菌的治疗干预的潜在目标.

关键词:

微生物组是一个微生物组.这是基因组解析的基因组.乳酸乳酸是一种乳酸.乳酸细菌是一种乳酸细菌.转换代码组学 (metatranscriptomics) 是一个鼠类动物 (murine) 是一种动物.

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