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来自大米土壤的硫酸盐减少细菌甲基化含有新型ArsSM融合蛋白质

Bao-Yun Yang¹, Chuan Chen¹, Axiang Gao¹

¹Jiangsu Key Laboratory for Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.

Environmental science & technology

|October 15, 2024

概括

厌氧细菌,特别是硫酸盐降解细菌,是微生物甲基化中的关键参与者. 在Solidesulfovibrio sp.中发现了一种新的双功能酶,ArsSM. TC1,催化甲基化中的关键步骤.

科学领域:

环境微生物学环境微生物学
生物地质化学生物地质化学
的新陈代谢的新陈代谢

背景情况:

微生物甲基化对于的生物地球化学至关重要.
关于在土中的甲基化微生物的知识有限.

研究的目的:

从大米土壤中分离和描述甲基化细菌.
研究由硫酸盐减少细菌甲甲基化的机制.
确定参与甲基化过程的新型酶.

主要方法:

从土中分离和选无氧和有氧细菌.
使用分离的细菌菌株进行甲基化试验.
在Solidesulfovibrio sp.中进行酶表征和基因鉴定. TC1.1. 这是一个很好的方法.

主要成果:

在86种无氧分离物中,有14种,包括7种低硫酸盐细菌 (SRB),甲基化化合物;没有一种有氧分离物显示出这种能力.
在Solidesulfovibrio sp.中发现了一种新的双功能酶 (ArsSM). TC1,融合了As(III) S-adenosylmethionine甲基转移酶 (ArsM) 和一个根基SAM蛋白.
ArsSM催化了As(III) 甲基化和随后的腺化,而SRB的高硫化度则抑制了MMA到DMA甲基化.

结论:

甲甲基化在厌氧细菌,特别是SRB中比在有氧细菌中更为普遍.
鉴定到的ArsSM酶及其在无氧生物中的广泛分布表明,它在无氧甲基化中起着重要作用.
了解这些微生物过程对于在受污染的环境中管理至关重要.

关键词:

亚是一种.甲基化的甲基化.亚酸盐 S-adenosylmethionine 甲基转移酶的作用.激进的SAM酶SAM激素是一种激素.减少硫酸盐的细菌减少硫酸盐的细菌

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