Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Topoisomerases02:02

DNA Topoisomerases

31.0K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
31.0K
DNA as a Genetic Template02:05

DNA as a Genetic Template

6.8K
6.8K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.1K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.1K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

9.9K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
9.9K
DNA Replication02:40

DNA Replication

48.9K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
48.9K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

14.0K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
14.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Computational optimization of MALT1 inhibitors against DLBCL: a QSAR-guided molecular docking and dynamics study.

In silico pharmacology·2025
Same author

Metal Exchange in Thioguanosine Coordination Polymers of Gold (I) and Silver (I).

Chemistry (Weinheim an der Bergstrasse, Germany)·2025
Same author

Protonolysis and Condensation Reactions of Alkoxido-Substituted Lindqvist {MW<sub>5</sub>} and Keggin {MPW<sub>11</sub>} Polyoxometalates: Comparative Experimental and Modeling Studies.

Inorganic chemistry·2025
Same author

Cheminformatics-based discovery of new organoselenium compounds with potential for the treatment of cutaneous and visceral leishmaniasis.

Journal of biomolecular structure & dynamics·2023
Same author

Vesicles, fibres, films and crystals: A low-molecular-weight-gelator [Au(6-thioguanosine)<sub>2</sub>]Cl which exhibits a co-operative anion-induced transition from vesicles to a fibrous metallo-hydrogel.

Soft matter·2023
Same author

Virtual screening and molecular dynamic simulations of the antimalarial derivatives of 2-anilino 4-amino substituted quinazolines docked against a <i>Pf</i>-DHODH protein target.

The Egyptian journal of medical human genetics·2023

相关实验视频

Updated: Jun 10, 2025

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

9.0K

在DNA分子上模拟铁 (III) 氧化物.

Siyaka Mj Zubairu1,2, Sulaiman O Idris2, Casmir E Gimba2

  • 1Chemical Nanoscience Laboratories, School of Natural and Environmental Sciences, Bedson Building, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.

Nanomaterials (Basel, Switzerland)
|October 15, 2024
PubMed
概括

研究人员使用DNA模板合成了铁 (III) 氧化物 (FeOOH和Fe2O3),创造了独特的树突性纳米结构. DNA模板影响了晶体大小和形态,FeOOH/DNA显示导电性.

关键词:
它们是DNA DNA DNA DNA.哥斯提人就是哥斯提人.血矿是血矿的一种.铁氧化物 铁氧化物模板设计 模板设计

更多相关视频

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
08:59

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

11.5K
Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation
12:03

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation

Published on: July 23, 2015

14.3K

相关实验视频

Last Updated: Jun 10, 2025

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

9.0K
DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
08:59

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

11.5K
Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation
12:03

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation

Published on: July 23, 2015

14.3K

科学领域:

  • 材料科学 材料科学 材料科学
  • 纳米技术 纳米技术
  • 生物材料是一种生物材料.

背景情况:

  • 氧化铁在各种应用中至关重要,但控制其纳米尺度形态和特性仍然具有挑战性.
  • DNA的自组装特性为模板纳米材料合成提供了一个有前途的途径.
  • 了解DNA模板和氧化铁形成之间的相互作用是开发新型功能材料的关键.

研究的目的:

  • 使用DNA作为模板合成铁 (III) 氧化 (FeOOH) 和铁 (III) 氧化物 (Fe2O3) 纳米粒子.
  • 描述合成材料的结构,光学和形态特性.
  • 为了研究DNA模板氧化铁的电导率.

主要方法:

  • 在存在或缺少DNA的情况下,Fe (III) 盐与NaOH的沉.
  • 对FeOOH和Fe2O3.3的合成进行控制的pH和温度.
  • 使用UV-Vis吸收,FTIR,X射线光电子光谱 (XPS),X射线衍射 (XRD),传输电子显微镜 (TEM) 和扫描导电显微镜 (SCM) 的表征.

主要成果:

  • 通过DNA模板合成FeOOH/DNA和Fe2O3/DNA材料取得了成功.
  • XRD证实了alpha-FeOOH和alpha-Fe2O3多态的形成,结晶体大小约为7纳米.
  • TEM揭示了针状晶体,这些晶体被组织成由DNA模拟的10微米级树突结构.
  • Fe2O3/DNA的光带间隙为3.2 eV,而FeOOH/DNA和Fe2O3/DNA的吸收较弱,与2 eV附近的间接间隙一致.
  • 在对齐后FeOOH/DNA显示导电性,而Fe2O3/DNA显示没有可检测的导电性.

结论:

  • DNA 作为一种有效的模板,用于合成FeOOH和Fe2O3.3的有序纳米结构.
  • DNA模板显著影响形态,导致树突组合.
  • FeOOH/DNA的导电性表明在纳米电子设备中的潜在应用,而Fe2O3/DNA缺乏导电性则需要进一步研究.