通过S2子单元替代物开发一种安全的,宽谱的减弱PEDV候选疫苗
Ding Zhang1,2, Yunfei Xie1,2, Qi Liao1,2
1State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Journal of virology
|October 15, 2024
概括
通过替代S2子单元,开发了一种新的仿真猪流行性腹病毒 (PEDV) 疫苗,YN-S2DR13. 这种新型PEDV疫苗显示病毒性降低,并提供针对G1和G2菌株的广泛保护.
科学领域:
- 兽医病毒学 兽医病毒学
- 疫苗开发 疫苗开发
- 分子生物学分子生物学
背景情况:
- 猪流行性腹 (PED) 在猪产业造成重大经济损失.
- 快速的PEDV基因组变异,特别是在尖端 (S) 蛋白中,限制了疫苗交叉保护.
- 开发安全,广泛,有效的减弱PEDV疫苗仍然是一个挑战.
研究的目的:
- 为了评估一种仿制PEDV菌株 (YN-S2DR13) 的致病性,组织热带性和免疫性.
- 评估S2亚单元替代的潜力,作为设计活体减弱PEDV疫苗的策略.
主要方法:
- 通过替换S蛋白的S2亚单元,构建一个模拟PEDV病毒 (YN-S2DR13).
- 评估新生猪YN-S2DR13的致病性和热带性.
- 免疫性评估,包括对断奶猪中PEDV G1和G2菌株的中和抗体诱导.
- 在体外传递以确定遗传稳定性.
主要成果:
- 仿真YN-S2DR13病毒失去了素的依赖性,并在Vero细胞中增加了传播.
- 与野生型YN菌株相比,YN-S2DR13在小猪中显著降低了毒性.
- 免疫接种YN-S2DR13诱导中和抗体,对PEDVG1和G2两种菌株都有效.
- 在体外研究证实了YN-S2DR13的遗传稳定性.
结论:
- 替代S2亚单元是快速开发活体减弱PEDV疫苗的可行策略.
- 仿真YN-S2DR13证明了其作为一种新型候选疫苗的潜力,可以提供广泛的保护.
- YN-S2DR13具有优势,包括没有素的高位传播和遗传稳定性.
相关概念视频
01:23Vaccine Production
Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
01:21Vaccines
Vaccines are among the most effective tools in preventive medicine, designed to prepare the immune system to recognize and combat infectious agents. By introducing antigens—substances that the immune system identifies as foreign—vaccines stimulate an adaptive immune response that leads to immunological memory. This immunological memory enables the body to mount a faster and more effective response upon future exposures to the actual pathogen.Vaccines can be categorized based on the type of...