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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

1.9K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
1.9K

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相关实验视频

Updated: May 2, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.8K

基于对称功率指数相的单分子追踪技术.

Famin Wang, Huijian Liu, Yunhai Zhang

    Optics letters
    |October 15, 2024
    PubMed
    概括

    我们开发了一种新的对称级指数阶段,用于先进的3D单分子成像. 这种方法可以实现精确的纳米尺度定位和跟踪,可调节探测深度.

    科学领域:

    • 光学和光子学 在光学和光子学.
    • 生物物理学的生物物理.
    • 纳米技术 纳米技术

    背景情况:

    • 准确的3D单分子定位对于理解生物过程至关重要.
    • 现有的方法在探测深度和精度方面存在局限性.
    • 阶段调制为改善点传播函数工程提供了一个有前途的途径.

    研究的目的:

    • 设计和验证一种新的相调节点分布函数,用于增强的3D纳米尺度定位.
    • 为了实现精确的3D单分子跟踪和定位成像,可调节轴向探测深度.

    主要方法:

    • 一个对称的指数级阶段的设计.
    • 开发了一个相调节的点分布函数,具有可转向的双主叶.
    • 截断过和基于相位逆转的优化算法的应用.
    • 在10微米轴深度使用多分子3D跟踪进行实验验证.

    主要成果:

    • 拟议的设计产生了一个点分布函数,其中有两个对称的主叶向光轴方向.
    • 通过操纵特定的设计参数来实现可调节的轴向探测深度.
    • 优化策略显著减少了侧叶和增强了定位精度.
    • 成功的多分子3D跟踪证明了该方法在10微米深度的有效性.

    更多相关视频

    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
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    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

    Published on: September 5, 2019

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    Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
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    Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

    Published on: November 23, 2021

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    相关实验视频

    Last Updated: May 2, 2026

    A Protocol for Real-time 3D Single Particle Tracking
    10:16

    A Protocol for Real-time 3D Single Particle Tracking

    Published on: January 3, 2018

    14.8K
    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
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    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

    Published on: September 5, 2019

    8.2K
    Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
    08:26

    Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

    Published on: November 23, 2021

    2.5K

    结论:

    • 对称功率指数相位设计为纳米级3D定位提供了一个强大的平台.
    • 这种方法显著提高了单分子3D跟踪和成像的能力.
    • 该方法提供可调节的轴向探测深度,增强其在生物应用中的多功能性.