在血液透析溶液中开发,验证和应用Na,K,Ca和Mg的快速序列方法,由HR-CS F AAS在血液透析溶液中进行
Flávia E Schwartzhaupt1, Leandro Kolling2,3, Wiliam Boschetti4
1Federal University of Rio Grande do Sul, Post-graduation Program in Pharmaceutical Sciences, Faculty of Pharmacy, Av. Ipiranga, 2752, Porto Alegre, RS 90610-000, Brazil.
Journal of AOAC International
|October 15, 2024
概括
一种新方法使用高分辨率连续源火焰原子吸收光谱学 (HR-CS F AAS) 准确确定血液透析溶液中的,,和. 这种方法大大减少了质量控制的分析时间.
科学领域:
- 分析化学 分析化学
- 频谱学是一种光谱学.
背景情况:
- 血液透析溶液对于脏替代疗法至关重要.
- 准确量化这些溶液中的电解质对于患者的安全至关重要.
研究的目的:
- 开发和验证一种用于测定血液透析溶液中的 (Na), (K), (Ca) 和 (Mg) 的新方法.
- 使用高分辨率连续源火焰原子吸收光谱学 (HR-CS F AAS) 结合实验设计 (DOE) 进行方法优化.
主要方法:
- 使用Doehlert矩阵和可取性函数优化关键变量 (乙烯流量,燃烧器高度,Cs和La度).
- 根据国际协调理事会 (ICH) 准则进行验证,评估选择性,线性,精度,准确性和稳定性.
主要成果:
- 精确 (RSD <2.7%) 和准确 (恢复100.5103.0%) 的Na,K,Ca和Mg的确定.
- 已建立的校准曲线在每个元素的特定度范围内.
- 在单个分析运行中成功进行了连续的多元件确定.
结论:
- 开发的HR-CS F AAS方法为血液透析溶液中的电解质分析提供了一种经过验证,高效和准确的方法.
- 与现有技术相比,这种方法可显著减少样品和标准准备时间.
- 介绍了一个新的替代品,目前的欧洲药典方法用于血液透析溶液分析.
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